Contemporary Methacrylate Resin-based Root Canal Sealers Exhibit Different Degrees of Ex Vivo Cytotoxicity When Cured in Their Self-cured Mode
Contemporary Methacrylate Resin-based Root Canal Sealers Exhibit Different Degrees of Ex Vivo Cytotoxicity When Cured in Their Self-cured Mode
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DOI：
10.1016/j.joen.2008.11.008
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发表时间：
2009-02-01
影响因子：
4.2
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
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作者：
Ames, Jason M.;Loushine, Robert J.;Tay, Franklin R.
通过3-（4，5-二甲基-噻唑基）-2，5-二苯基-溴化四唑鎓试验研究了四种甲基丙烯酸酯树脂密封剂的细胞毒性，该试验通过评估其琥珀酸脱氢酶活性来测量细胞活力。密封剂在自固化模式下聚合，以模拟其挤出到根周组织中时的凝固条件。使用EndoREZ（Ultradent，South Jordan，UT）、RealSeal（SybronEndo，橙子，CA）、MetaSEAL（Parkell，法明顿，NY）和RealSeal SE（SybronEndo）以及阳性和阴性对照品制备了生物样品。固定后，将它们与大鼠骨肉瘤（ROS 17/2.8）细胞直接接触，并在模拟体液（SBF）中浸泡5周。所有密封剂最初（第0周）均表现出严重毒性。EndoREZ和RealSeal在SBF浸泡5个周期后仍具有严重毒性。MetaSEAL和RealSeal SE两种自酸蚀树脂基封闭剂的毒性随时间逐渐降低。暴露于RealSeal SE的细胞的透射电子显微镜显示不同程度的细胞损伤，反映了其毒性状态。具有完整线粒体的细胞在第5周时变得无细胞毒性后可识别。（J Endod 2009;35：225-228）
The cytotoxicity of four methacrylate resin-based sealers was investigated by the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay, which measures cell viability by assessing its succinate dehydrogenase activity. The sealers were polymerized in the self-cured mode to Simulate the setting condition upon their extrusion into periradicular tissues. Disks were prepared from EndoREZ (Ultradent, South Jordan, UT), RealSeal (SybronEndo, Orange, CA), MetaSEAL (Parkell, Farmington, NY), and RealSeal SE (SybronEndo) together with positive and negative controls. After setting, they were placed in direct contact with rat osteosarcoma (ROS 17/2.8) cells and for 5 succeeding weeks after immersing in simulated body fluid (SBF). All sealers exhibited severe toxicity initially (week 0). EndoREZ and RealSeal remained severely toxic after five cycles of SBF immersion. Toxicity of the two self-etching resin-based sealers MetaSEAL and RealSeal SE decreased gradually over time. Transmission electron microscopy of cells exposed to RealSeal SE showed variable degrees of cell injury that reflect its toxicity status. Cells with intact mitochondria were identifiable after the sealer became non-cytotoxic at week 5. (J Endod 2009;35:225-228)