Immunogold localization of circumsporozoite protein of the malaria parasite Plasmodium falciparum during sporogony in Anopheles stephensi midguts.
Immunogold localization of circumsporozoite protein of the malaria parasite Plasmodium falciparum during sporogony in Anopheles stephensi midguts.
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史氏按蚊中肠孢子发生期间疟原虫恶性疟原虫的环子孢子蛋白的免疫金定位。
DOI：
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发表时间：
1988
影响因子：
6.6
通讯作者：
H. Geuze
中科院分区：
文献类型：
--
作者：
G. Posthuma;Jacques F. Meis;J. Verhave;M. Hollingdale;T. Ponnudurai;J. Meuwissen;H. Geuze
The occurrence of the circumsporozoite (CS) proteins of Plasmodium falciparum sporozoites was monitored during sporogonic development in Anopheles stephensi mosquitoes. Using a monoclonal anti-CS protein antibody (3Sp2) and immunogold labeling on ultrathin cryosections it was found that CS protein is synthesized in immature oocysts from day 6 onwards when there are not yet signs of sporozoite formation. The CS protein is rapidly incorporated in the oocyst plasmalemma, which subsequently invaginates into the parasite. In the oocyst only the external sporozoite membrane contains CS protein. The inner pellicle membranes, rhoptries and micronemes do not react with monoclonal antibody (MoAb) 3Sp2.
