Rapid detection of Bacillus spore aerosol particles by direct in situ analysis using MALDI-TOF mass spectrometry
Rapid detection of Bacillus spore aerosol particles by direct in situ analysis using MALDI-TOF mass spectrometry
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DOI：
10.1111/lam.12261
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发表时间：
2014-08-01
影响因子：
2.4
通讯作者：
Kim, S. -J.
中科院分区：
文献类型：
--
作者：
Jeong, Y. -S.;Choi, S.;Kim, S. -J.
准确、快速的分析方法对于检测和鉴定生物战剂至关重要。尽管各种研究已经调查了使用MALDI-TOF MS进行细菌分类，但只有少数研究检查了用于鉴定BWAs的方法的适用性。本研究旨在产生、收集和分析2-10 μ m的芽孢杆菌孢子气溶胶颗粒，这是BWA的最佳尺寸。使用直接原位MALDI-TOF MS快速分析2-10 μ m的芽孢杆菌孢子气溶胶颗粒，无需预处理过程。成功地检测到芽孢杆菌孢子气溶胶颗粒的质谱。为了实现芽孢杆菌孢子的实时检测和鉴定，建立了芽孢杆菌孢子的质谱数据库，并开发了相应的算法。利用质谱技术对芽孢杆菌孢子颗粒进行了快速检测和鉴定，可用于生化武器的检测和检验。
Accurate and rapid analytical methods are essential for the detection and identification of biological warfare agents (BWA). Although various studies have investigated the use of MALDI-TOF MS for bacterial classification, only a few studies have examined the applicability of method for the identification of BWAs. This study aimed to generate, collect and analyse Bacillus spore aerosol particles of 2-10 mu m, the optimal size of a BWA. Bacillus spore aerosol particles of 2-10 mu m were rapidly analysed using direct in situ MALDI-TOF MS without pretreatment processes. A mass spectrum of Bacillus spore aerosol particles was successfully detected. For real-time detection and identification, a mass spectral database of Bacillus spores was constructed, and an algorithm was developed and applied. Bacillus spore particles were rapidly detected and identified by MS, which can be used for the detection and inspection of BWAs.