Induction and upregulation by interleukin 2 of high-affinity interleukin 2 receptors on thymocytes and T cells.
Induction and upregulation by interleukin 2 of high-affinity interleukin 2 receptors on thymocytes and T cells.
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DOI：
10.1073/pnas.82.24.8663
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发表时间：
1985-12
影响因子：
11.1
通讯作者：
G. H. Reem;N. Yeh;D. Urdal;P. Kilian;J. Farrar
中科院分区：
文献类型：
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作者：
G. H. Reem;N. Yeh;D. Urdal;P. Kilian;J. Farrar
我们发现，纯化的重组白介素2(rIL-2)单独在体外诱导高亲和力和低亲和力的白介素2(IL-2)受体在人T细胞和胸腺细胞上的表达，而这些受体以前没有被凝集素或其他诱导剂激活。IL-2受体在24小时后表达，通过125I标记的抗IL-2受体单抗2A3的结合来确定，该抗体同样与高亲和力和低亲和力受体结合。高亲和力受体与低亲和力受体的区别在于125I标记的IL-2与T细胞的结合，以及胸腺细胞对IL-2的增殖反应，其浓度选择性地与高亲和力类别的IL-2受体相互作用。IL-2单独诱导胸腺细胞的增殖依赖于IL-2的浓度，并可被抗Tac单抗抑制，提示该增殖反应是由IL-2与受体结合介导的。此外，我们还证明了IL-2可以增加刀豆蛋白A激活的胸腺细胞上高亲和力受体的数量。这些结果证明，IL-2作为一种激素，诱导胸腺细胞和T细胞的激活，从生物活性的、高亲和力的IL-2受体的从头诱导中得到了证明。IL-2还上调活化胸腺细胞高亲和力IL-2受体的表达。这些观察结果说明了IL-2在免疫反应中调节作用的生物学重要性。
We show that purified recombinant interleukin 2 (rIL-2) alone induces the expression of high- and low-affinity interleukin 2 (IL-2) receptors in vitro on human T cells and thymocytes that have not been activated previously by lectins or other inducing agents. IL-2 receptors are expressed after 24 hr, as determined by the binding of 125I-labeled monoclonal anti-IL-2 receptor antibody 2A3, which binds equally to high- and low-affinity receptors. High-affinity receptors were distinguished from low-affinity receptors by the binding of 125I-labeled IL-2 to T cells and by the proliferative response of thymocytes to IL-2, in concentrations that selectively interact with the high-affinity class of IL-2 receptors. The IL-2-induced proliferation of thymocytes in vitro induced by IL-2 alone is dependent upon the concentration of IL-2 and is inhibited by monoclonal anti-Tac antibody, indicating that the proliferative response is mediated by the binding of IL-2 to the receptors. In addition, we demonstrate that IL-2 augments the number of high-affinity receptors on concanavalin A-activated thymocytes. These results document that IL-2 acts as a hormone that induces the activation of thymocytes and T cells, as evidenced by the de novo induction of biologically active, high-affinity IL-2 receptors. IL-2 also upregulates the expression of high-affinity IL-2 receptors on activated thymocytes. These observations illustrate the biologic importance of the regulatory role of IL-2 in the immune response.