Apparent inter-channel interference in dual-electrode electrochemical detection.
Apparent inter-channel interference in dual-electrode electrochemical detection.
复制标题
双电极电化学检测中明显的通道间干扰。
DOI：
10.1016/0021-9673(92)80210-l
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Dunn,AJ
中科院分区：
文献类型：
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作者：
Dempsey,CA;Lavicky,J;Dunn,AJ
在使用双电极电化学检测器对脑儿茶酚胺进行常规高效液相色谱分析的过程中，我们在较低电压通道中遇到了一个不寻常的负峰。随后的研究表明，该峰是由酪氨酸引起的，酪氨酸在高压通道中产生正峰。我们的研究表明，在一个通道中产生峰的化合物似乎是负责在第二个通道中设置在较低的电压，接近或低于氧化所需的复杂的峰。复合峰是双相的;尖锐的负峰与较高电压通道上的正峰重合，随后是正峰。这种效应对酪氨酸不是特异性的，但在低压通道上观察到，所有测试的化合物在高压通道上产生信号。该问题的原因尚不清楚，但似乎是双通道系统中两个电极通道电耦合的伪影。
During the course of routine high-performance liquid chromatographic analyses of brain catecholamines using dual-electrode electrochemical detection, we encountered an unusual negative peak in the lower-voltage channel. Subsequent investigations suggested that this peak was caused by tyrosine which produced a positive peak in the higher-voltage channel. Our investigations indicate that compounds that generate a peak in one channel appear to be responsible for complex peaks in a second channel set at a lower voltage, close to or below that necessary for oxidation. The complex peaks are biphasic; a sharp negative peak coinciding with the positive peak on the higher-voltage channel, followed by a positive peak. This effect was not specific for tyrosine, but was observed on the lower-voltage channel with all compounds tested that produced signals on the high-voltage channel. The cause of the problem is unknown, but it appears to be an artifact of the electrical coupling of the two electrode channels in a dual-channel system.