Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor
Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor
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DOI：
10.1093/emboj/21.6.1360
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发表时间：
2002-03-15
期刊：
影响因子：
11.4
通讯作者：
Inzé, D
中科院分区：
文献类型：
--
作者：
De Veylder, L;Beeckman, T;Inzé, D
新的植物细胞出现在分生组织，在那里它们分裂几次，然后离开细胞周期程序并开始分化。在这里，我们表明，E2 Fa-DPa转录因子的拟南芥是一个关键的调节器，决定植物细胞的增殖状态。E2 Fa的异位表达诱导正常分化的子叶和下胚轴细胞的持续细胞增殖。E2 Fa与其二聚化伴侣DPa的共表达强烈增强了表型。在核内复制细胞中，E2 Fa-DPa还引起与S期基因的转录诱导相关的额外DNA复制。由于E2 Fa-DPa转基因植物在发育早期被抑制，我们认为细胞周期的受控退出是植物正常发育的先决条件。
New plant cells arise at the meristems, where they divide a few times before they leave the cell-cycle program and start to differentiate. Here we show that the E2Fa-DPa transcription factor of Arabidopsis thaliana is a key regulator determining the proliferative status of plant cells. Ectopic expression of E2Fa induced sustained cell proliferation in normally differentiated cotyledon and hypocotyl cells. The phenotype was enhanced strongly by the co-expression of E2Fa with its dimerization partner, DPa. In endoreduplicating cells, E2Fa-DPa also caused extra DNA replication that was correlated with transcriptional induction of S phase genes. Because E2Fa-DPa transgenic plants arrested early in development, we argue that controlled exit of the cell cycle is a prerequisite for normal plant development.