MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
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DOI：
10.1515/cclm.2009.126
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发表时间：
2009-05-01
影响因子：
6.8
通讯作者：
Lee, Hye Seung
中科院分区：
文献类型：
--
作者：
Park, Kyoung Un;Lee, Hee Eun;Lee, Hye Seung
Background: Detection of tumor-associated genetic alterations in plasma of cancer patients has recently been suggested to be an accurate method for detecting early or recurrent cancer.Methods: We performed quantitative real-time PCR for MYC and GAPDH in tissue and plasma samples of 57 patients with gastric cancer and in plasma of 79 cancer-free individuals. We also performed two-color MYC fluorescence in situ hybridization (FISH) in tissue from the 57 patients with gastric cancer.Results: The tissue MYC/GAPDH ratio by real-time PCR was significantly correlated with MYC status by FISH (p
