Hemoglobin-Loaded Triblock PCLA-PEG-PCLA Copolymer Vesicles: A Potential Oxygen Carrier
Hemoglobin-Loaded Triblock PCLA-PEG-PCLA Copolymer Vesicles: A Potential Oxygen Carrier
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负载血红蛋白的三嵌段 PCLA-PEG-PCLA 共聚物囊泡：潜在的氧载体
DOI：
10.1166/jnn.2016.11342
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发表时间：
2016-07
影响因子：
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通讯作者：
Zhiyong Qian
中科院分区：
文献类型：
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作者：
Qian Yang;Xiujun Yu;Tao Li;Wenting Li;Qinglin Jiang;Jun Zhang;Zhiyong Qian
载血红蛋白囊泡(HB-vesicles)作为细胞型血红蛋白氧载体(HBOCs)已被开发了多年。通过改进的水包油复乳法制备了一种新型的两亲三嵌段共聚物，即聚(ε-己内酯-共丙交酯)-聚乙二醇聚(ε-己内酯-共丙交酯)-聚己内酯-聚乳酸两亲三嵌段共聚物。在W/O/W包埋过程中，血红蛋白(Hb)被转移到一氧化碳血红蛋白(CO-Hb)上以稳定Hb。测定了所制备的CO-HB-囊泡的包封率和粒径分布。对包埋条件进行优化后，粒径为180 nm的CO-HB-囊泡包封率最高可达67.5%，显示出作为长循环载氧载体的潜力。最后，在氧气气氛中的可见光照射下，囊泡中的CO-Hb可以在很短的时间内转化为氧结合血红蛋白(O2-Hb)。所得的HB-囊泡表现出与天然红细胞相似的氧亲和力，表明该纳米系统可以作为一种有前途的HBOC。
Hemoglobin-loaded vesicles (Hb-Vesicles) have been developed for several years as cellular-type hemoglobin-based oxygen carriers (HBOCs). We in the present study reported a new type of Hb-Vesicles that were easily fabricated by poly(ε-caprolactone-co- lactide)-poly(ethylene glycol)-poly(ε-caprolactone-co-lactide) (PCLA-PEG-PCLA) amphiphilic triblock copolymers via an improved water-in-oil-in-water (W/O/W) double emulsion method. Hemoglobin (Hb) was transferred to carbonylhemoglobin (CO–Hb) for Hb stabilization during the W/O/W encapsulation. The encapsulation efficiency and size distribution of the obtained CO–Hb-Vesicles were determined. The highest CO–Hb-Vesicles encapsulation efficiency could reach 67.5%with 180 nm particle diameters after optimization of encapsulation conditions, showing promising potential as long-circulating oxygen carriers. Finally, the CO–Hb in the vesicles could be converted to oxygen-binding hemoglobin (O2-Hb) at a very short time by irradiation of visible light in oxygen atmosphere. The resultant Hb-Vesicles exhibited similar oxygen affinity to the native red blood cells demonstrating that this nano-system can serve as a promising HBOC.