Three-dimensional measurement of alveolar airspace volumes in normal and emphysematous lungs using micro-CT
Three-dimensional measurement of alveolar airspace volumes in normal and emphysematous lungs using micro-CT
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DOI：
10.1152/japplphysiol.91227.2008
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发表时间：
2009-08-01
影响因子：
3.3
通讯作者：
Suki, Bela
中科院分区：
文献类型：
--
作者：
Parameswaran, Harikrishnan;Bartolak-Suki, Erzsebet;Suki, Bela
pareswaran H, Bartolak-Suki E, Hamakawa H, Majumdar A, Allen PG, Suki B.微ct对正常肺气肿肺肺泡空间体积的三维测量。中国生物医学工程学报（英文版），2009；首次发表于2009年6月18日；doi: 10.1152 / japplphysiol.91227.2008。在肺气肿中，肺泡结构通过三维（3D）过程逐渐破裂，导致空气空间扩大。然而，这种结构变化的表征是基于二维（2D）组织切片的测量或基于二维测量的3D结构估计。在这项研究中，我们开发了一种新的银染色方法，用于使用显微计算机断层扫描（CT）成像在3D中显示组织结构，结果表明，在30 cmH(2)O固定压力下，在弹性酶诱导损伤后7天和14天，肺泡空间的平均体积分别从正常小鼠的0.12 nl增加到肺气肿小鼠的0.44 nl和2.14 nl。我们还使用激光扫描共聚焦显微镜评估了二维组织结构。肺泡间隙等效直径的平均值在二维组中低于三维组，而其方差在二维组中高于三维组。然而，正常小鼠和肺气肿小鼠肺泡空间大小的2D和3D统计比较结果相似：与对照组相比，治疗后7天等效直径的平均值和方差均增加。这些指标在治疗后第7 ~ 14天进一步升高。在治疗后的前7天，SD的相对变化速度比平均等效直径的相对变化要快得多。我们的结论是，量化结构的异质性可以为肺气肿的发病机制或进展提供新的见解，这可以通过使用3D测量提高灵敏度来增强。
Parameswaran H, Bartolak-Suki E, Hamakawa H, Majumdar A, Allen PG, Suki B. Three-dimensional measurement of alveolar airspace volumes in normal and emphysematous lungs using micro-CT. J Appl Physiol 107: 583-592, 2009. First published June 18, 2009; doi:10.1152/japplphysiol.91227.2008.-In pulmonary emphysema, the alveolar structure progressively breaks down via a three-dimensional (3D) process that leads to airspace enlargement. The characterization of such structural changes has, however, been based on measurements from two-dimensional (2D) tissue sections or estimates of 3D structure from 2D measurements. In this study, we developed a novel silver staining method for visualizing tissue structure in 3D using micro-computed tomographic (CT) imaging, which showed that at 30 cmH(2)O fixing pressure, the mean alveolar airspace volume increased from 0.12 nl in normal mice to 0.44 nl and 2.14 nl in emphysematous mice, respectively, at 7 and 14 days following elastase-induced injury. We also assessed tissue structure in 2D using laser scanning confocal microscopy. The mean of the equivalent diameters of the alveolar airspaces was lower in 2D compared with 3D, while its variance was higher in 2D than in 3D in all groups. However, statistical comparisons of alveolar airspace size from normal and emphysematous mice yielded similar results in 2D and 3D: compared with control, both the mean and variance of the equivalent diameters increased by 7 days after treatment. These indexes further increased from day 7 to day 14 following treatment. During the first 7 days following treatment, the relative change in SD increased at a much faster rate compared with the relative change in mean equivalent diameter. We conclude that quantifying heterogeneity in structure can provide new insight into the pathogenesis or progression of emphysema that is enhanced by improved sensitivity using 3D measurements.