Role of IL-10 polymorphisms in susceptibility to hepatitis B virus-related hepatocellular carcinoma
Role of IL-10 polymorphisms in susceptibility to hepatitis B virus-related hepatocellular carcinoma
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IL-10多态性在乙型肝炎病毒相关肝细胞癌易感性中的作用
DOI：
10.4238/gmr.15017984
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发表时间：
2016
影响因子：
0.4
通讯作者：
C.Y. Dong1
中科院分区：
文献类型：
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作者：
M.W. Peng;S.Q. Lu;J. Liu;C.Y. Dong1
抽象的。我们进行了一项病例对照研究，以探讨IL-10三种常见的单核苷酸多态(-592G/A、-819T/C和-1082A/C)在乙型肝炎病毒(HBV)相关性肝细胞癌(HCC)发生中的作用。这项研究包括173名与乙肝相关的肝细胞癌患者和182名健康对照。应用聚合酶链式反应-限制性片段长度多态性分析来评估感兴趣的序列变体。与对照组相比，肝癌患者年龄更大(t=1.94，P=0.03)，有癌症家族史(卡方=17.86，P<；0.001)，丙氨酸转氨酶(t=13.32，P<；0.001)和天冬氨酸转氨酶(t=12.63，P<；0.001)水平较高。非条件Logistic回归分析发现，与AA基因型相比，-592G/A的GG基因型与肝癌的风险增加相关[优势比(OR)=2.20，95%可信区间(CI)=1.12~4.38]。在显性模式下，AG+GG基因与AA基因相比与乙肝相关肝细胞癌的易感性相关，OR(95%CI)为1.56(1.02-2.48)。此外，隐性模型显示，与AA+AG基因型相比，GG基因型与肝癌风险增加相关(OR=1.85，95%CI=1.01~3.47)。然而，在共显性、显性和隐性模式下，未观察到-819T/C和-1082A/C变异与乙肝相关肝细胞癌的发生相关。我们的结论是，IL-10-592G/A多态性在共显性、显性和隐性模式下确实在乙肝相关性肝细胞癌的易感性中起作用。
ABSTRACT. We conducted a case-control study to investigate the role of three common single nucleotide polymorphisms of IL-10 (-592G/A, -819T/C, and -1082A/C) in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The study included 173 HBV-related HCC patients and 182 healthy controls. A polymerase chain reaction-restriction fragment length polymorphism assay was applied to assess the sequence variants of interest. Compared with control subjects, HCC patients were more likely to be older (t = 1.94, P = 0.03), have a family history of cancer (chi square = 17.86, P < 0.001), and exhibit higher alanine transaminase (t = 13.32, P < 0.001) and aspartate transaminase (t = 12.63, P < 0.001) levels. Using unconditional logistic regression analyses, we found that the GG genotype of -592G/A was associated with increased risk of HCC [odds ratio (OR) = 2.20, 95% confidence interval (CI) = 1.12-4.38], compared to the AA genotype. Under a dominant model, the AG+GG genotype correlated with HBV-related HCC susceptibility compared to the AA genotype, with an OR (95%CI) of 1.56 (1.02-2.48). Moreover, a recessive model showed the GG genotype to be associated with elevated risk of HCC compared to the AA+AG genotype (OR = 1.85, 95%CI = 1.01-3.47). However, no significant.association between the -819T/C and -1082A/C variants and development of HBV-related HCC was observed under codominant, dominant, and recessive models. We conclude that the IL-10 -592G/A polymorphism does play a role in susceptibility to HBV-related HCC under codominant, dominant, and recessive models.