Hybrid Plasmonic Photoreactors as Visible Light-Mediated Bactericides
Hybrid Plasmonic Photoreactors as Visible Light-Mediated Bactericides
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DOI：
10.1021/acsami.9b14834
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发表时间：
2020-01-08
影响因子：
9.5
通讯作者：
Reinhard, Bjorn M.
中科院分区：
文献类型：
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作者：
An, Xingda;Naowarojna, Nathchar;Reinhard, Bjorn M.
光催化化合物和复合物，例如三(联吡啶)钌(II)、[Ru(bpy)(3)](2+)，最近作为光介导杀菌剂引起了人们的关注，有助于满足新抗菌策略的需求。我们在这项工作中证明，通过与等离子体纳米天线结合，可以显着增强[Ru(bpy)(3)](2+)的杀菌功效及其抗菌功能的控制。我们报告了通过纳米复合材料设计实现了强可见光控制的细菌灭活，该设计将 [Ru(bpy)(3)](2+) 作为光催化剂，将 Ag 纳米颗粒 (NP) 核心作为聚光纳米天线，纳入等离子体混合光反应器中。混合光反应器平台由封装银纳米粒子并结合光催化剂的自组装脂质膜促进。在没有共振照明的情况下，脂质膜使纳米复合材料具有生物相容性。光照后，[Ru(bpy)(3)](2+) 的金属到配体电荷转移带的等离子体增强光激发准备了复合物的反应激发态，氧化纳米复合膜并增加其渗透性。光氧化诱导[Ru(bpy)(3)](2+)、Ag+和过氧化脂质释放到环境介质中，它们在环境介质中协同相互作用以灭活细菌。我们测量到革兰氏阳性节杆菌减少了 7 个数量级。可见光照射 1 小时后，使用光反应器杀菌剂的革兰氏阴性大肠杆菌菌落形成单位减少了 4 个数量级。在这两种情况下，光反应器都超过了对数减少值 3 的杀菌标准，并且超过了游离 Ag NP 或 [Ru(bpy)(3)](2+) 的抗菌效果 > 4 个数量级。我们还在概念验证研究中实现了细菌薄膜的灭活。
Photocatalytic compounds and complexes, such as tris(bipyridine)ruthenium(II), [Ru(bpy)(3)](2+), have recently attracted attention as light-mediated bactericides that can help to address the need for new antibacterial strategies. We demonstrate in this work that the bactericidal efficacy of [Ru(bpy)(3)](2+) and the control of its antibacterial function can be significantly enhanced through combination with a plasmonic nanoantenna. We report strong, visible light-controlled bacterial inactivation with a nanocomposite design that incorporates [Ru(bpy)(3)](2+) as a photocatalyst and a Ag nanoparticle (NP) core as a light-concentrating nanoantenna into a plasmonic hybrid photoreactor. The hybrid photoreactor platform is facilitated by a self-assembled lipid membrane that encapsulates the Ag NP and binds the photocatalyst. The lipid membrane renders the nanocomposite biocompatible in the absence of resonant illumination. Upon illumination, the plasmon-enhanced photoexcitation of the metal-to-ligand charge-transfer band of [Ru(bpy)(3)](2+) prepares the reactive excited state of the complex that oxidizes the nanocomposite membrane and increases its permeability. The photooxidation induces the release of [Ru(bpy)(3)](2+), Ag+, and peroxidized lipids into the ambient medium, where they interact synergistically to inactivate bacteria. We measured a 7 order of magnitude decrease in Gram-positive Arthrobacter sp. and a 4 order of magnitude decrease in Gram-negative Escherichia coli colony forming units with the photoreactor bactericides after visible light illumination for 1 h. In both cases, the photoreactor exceeds the bactericidal standard of a log reduction value of 3 and surpasses the antibacterial effect of free Ag NPs or [Ru(bpy)(3)](2+) by >4 orders of magnitude. We also implement the inactivation of a bacterial thin film in a proof-of-concept study.