Immobilization of bovine serum albumin onto porous polyethylene membranes using strongly attached polydopamine as a spacer.
Immobilization of bovine serum albumin onto porous polyethylene membranes using strongly attached polydopamine as a spacer.
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DOI：
10.1016/j.colsurfb.2011.03.027
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发表时间：
2011-08
期刊：
影响因子：
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通讯作者：
Li-ping Zhu;Jin-Hong Jiang;Bao-ku Zhu;You-yi Xu
中科院分区：
文献类型：
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作者：
Li-ping Zhu;Jin-Hong Jiang;Bao-ku Zhu;You-yi Xu
基于多巴胺在水溶液中的自聚合和强粘附特性，开发了一种新颖而方便的将蛋白质固定在多孔聚乙烯（PE）膜上的方法。通过简单地将膜浸入多巴胺水溶液中一段时间，形成薄的聚多巴胺（pDA）层并将其紧密地涂覆在PE膜上。随后，通过牛血清白蛋白（BSA）与反应性聚多巴胺层之间的偶联，将BSA结合到所得的PE/pDA复合膜上。用衰减全反射-傅里叶变换红外光谱（ATR-FTIR）和X射线光电子能谱（XPS）对聚多巴胺层和BSA的牢固固定进行了验证。水接触角测定结果表明，聚多巴胺包覆并结合BSA后，PE膜的亲水性得到显著提高。血小板粘附实验表明，BSA固定化PE膜的血液相容性优于未改性PE膜和PE/pDA复合膜。对肝细胞培养和细胞活力的研究表明，聚多巴胺涂层赋予PE膜显着改善的细胞相容性。与BSA表面相比，聚多巴胺表面更有利于细胞的粘附、生长和增殖。
Based on the self-polymerization and strong adhesion characteristics of dopamine in aqueous solution, a novel and convenient approach was developed to immobilize protein onto porous polyethylene (PE) membranes. A thin polydopamine (pDA) layer was formed and tightly coated onto PE membrane by dipping simply the membrane into dopamine aqueous solution for a period of time. Subsequently, bovine serum albumin (BSA) was bound onto the obtained PE/pDA composite membranes via the coupling between BSA and the reactive polydopamine layer. The firm immobilization of polydopamine layer and BSA was verified by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results of water contact angle measurement showed that the hydrophilicity of PE membrane was significantly improved after coating polydopamine and binding BSA. The experiments of blood platelet adhesion indicated that BSA-immobilized PE membrane had better blood compatibility than the unmodified PE and the PE/pDA composite membranes. The investigations on hepatocyte cultures and cell viability revealed that the polydopamine coating endowed PE membrane with significantly improved cell compatibility. Compared to BSA surface, polydopamine surface is more favorable for cell adhesion, growth, and proliferation.