Effect of mild hypothermia on the expression of aquaporin family in cultured rat astrocytes under hypoxic condition
Effect of mild hypothermia on the expression of aquaporin family in cultured rat astrocytes under hypoxic condition
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DOI：
10.1016/j.neures.2003.08.006
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发表时间：
2003-12-01
影响因子：
2.9
通讯作者：
Asai, K
中科院分区：
文献类型：
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作者：
Fujita, Y;Yamamoto, N;Asai, K
水通道蛋白（Aquaporins，AQP）是一类与透镜主要内在蛋白（major intrinsic protein，MIP）同源的水选择性转运蛋白，在分泌和吸收细胞中增加质膜透性。本研究观察了亚低温对缺氧条件下培养的大鼠星形胶质细胞AQP 4、AQP 5和AQP 9表达的影响。在37 ℃时，观察到AQP 4、AQP 5和AQP 9 mRNA的表达显著降低。而在32 ℃（亚低温）时，AQP 5 mRNA的表达恢复到基础水平。有趣的是，在亚低温下，AQP 4 mRNA表达短暂下降，然后增加约2倍;而AQP 9 mRNA表达下降与37 ℃相同。Western blot分析证实了AQP 4和AQP 9蛋白表达的变化。AQP 4和AQP 5表达在32 ℃时由37 ℃缺氧诱导的下降恢复，可能在低温条件下减轻脑水肿中起重要作用。(C)2003年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
Aquaporins (AQPs) are a family of water-selective transporting proteins with homology to the major intrinsic protein (MIP) of lens, that increase plasma membrane water permeability in secretory and absorptive cells. In this study, we investigated the effect of mild hypothermia on the expression of AQP4, AQP5 and AQP9 in rat astrocytes cultured under hypoxic conditions. At 37degreesC, a marked decrease in the expression of AQP4, AQP5 and AQP9 mRNAs was observed. However, at 32degreesC (mild hypothermia), the expression of AQP5 mRNA was restored to its basal level. Interestingly, under mild hypothermia AQP4 mRNA expression transiently decreased and then increased about two-fold; while AQP9 mRNA expression decreased the same as at 37degreesC. The changes in the expression of AQP4 and AQP9 proteins were confirmed by Western blot analysis. The restoration of the AQP4 and AQP5 expression at 32degreesC from the hypoxia-induced decrease at 37degreesC may play an important role in the reduction of brain edema under hypothermic conditions. (C) 2003 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.