Temporal sequence of cell shape changes in cultured rat sertoli cells after experimental elevation of intracellular cAMP.
Temporal sequence of cell shape changes in cultured rat sertoli cells after experimental elevation of intracellular cAMP.
复制标题
细胞内 cAMP 实验性升高后，培养的大鼠支持细胞中细胞形状变化的时间序列。
DOI：
10.1016/0014-4827(81)90414-6
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发表时间：
1981
影响因子：
3.7
通讯作者：
Kierszenbaum,AL
中科院分区：
文献类型：
--
作者：
Spruill,WA;White,MG;Steiner,AL;Tres,LL;Kierszenbaum,AL
FSH和药理学试剂诱导培养的大鼠支持细胞的形状变化的能力已经通过使用延时相差显微镜和扫描电子显微镜（SEM）进行了研究。形态学研究结合免疫细胞化学方法定位cAMP在Sertoli细胞和相关的结果与蛋白结合cAMP在Sertoli细胞的测定。暴露于FSH、异丁基甲基黄嘌呤（MIX）、二丁酰环腺苷酸（db-cAMP）和FSH-MIX混合物后，不同数量的支持细胞从扁平的上皮样形态转化为星状形态。形态学变化遵循时间和生物制剂依赖性的改变和恢复模式。当120分钟暴露于FSH诱导38%的细胞发生形状变化时，MIX、db-cAMP和FSH-MIX影响75%的细胞发生形状变化。由MIX，db-cAMP和FSH-MIX诱导的形态转换持续，只要这些生物制剂存在于培养基中，而FSH单独诱导的效果是短暂的。在平面到星状过渡之前，细胞内蛋白结合的cAMP增加，环核苷酸的一种形式，可以解释在形态学响应和非响应支持细胞中观察到的cAMP免疫反应性。从这些数据和以前的实验结果的雄激素结合蛋白（ABP）的免疫反应性在细胞质中的反应性和非反应性支持细胞，我们得出结论，cAMP的激增触发一个尚未确定的机制，支持细胞修改它们的形状一致的细胞质分泌颗粒的逐步耗尽。
The ability of FSH and pharmacological agents to induce changes in the shape of cultured rat Sertoli cells has been studied by using time-lapse phase-contrast microscopy and scanning electron microscopy (SEM). Morphological studies were combined with an immunocytochemical method for the localization of cAMP in Sertoli cells and the results correlated with determinations of protein-bound cAMP in Sertoli cells. A variable number of Sertoli cells were converted from a flat, epithelial-like morphology into a stellate morphology after exposure to FSH, isobutyl-methylxanthine (MIX), dibutyryl cyclic AMP (db-cAMP) and an FSH-MIX mixture. The morphological changes followed a time- and biological agent-dependent alteration and recovery pattern. While a 120 min exposure to FSH induced shape changes in 38% of the cells, MIX, db-cAMP and FSH-MIX effected shape changes in 75 % of cells. The morphological conversion induced by MIX, db-cAMP and FSH-MIX persisted as long as these biological agents were present in the medium, whereas the effects induced by FSH alone were transient. The flat-to-stellate transition was preceded by an increase in intracellular protein-bound cAMP, a form of cyclic nucleotide which may account for cAMP immunoreactivity observed in morphologically responsive and non-responsive Sertoli cells. From these data and from previous experimental findings of androgen-binding protein (ABP) immunoreactivity in the cytoplasm of responsive and non-responsive Sertoli cells, we conclude that a surge of cAMP triggers a still undefined mechanism by which Sertoli cells modify their shape in coincidence with a progressive depletion of cytoplasmic secretory granules.