Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
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DOI：
10.3791/56124
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发表时间：
2017-08-01
影响因子：
1.2
通讯作者：
Hoffmann, Ary A.
中科院分区：
文献类型：
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作者：
Ross, Perran A.;Axford, Jason K.;Hoffmann, Ary A.
实验感染了沃尔巴克氏菌的埃及伊蚊被用于控制登革热、基孔肯雅热和寨卡病毒等虫媒病毒的传播。感染沃尔巴克氏菌的蚊子可以被释放到田间，通过不相容的交配来减少种群规模，或者用不易传播病毒的蚊子来改变种群。为了使这些策略取得成功，从实验室释放到野外的蚊子必须与本地蚊子竞争。然而，将蚊子留在实验室可能会导致近亲繁殖、遗传漂移和实验室适应，这可能会降低它们在野外的适应能力，并可能扰乱实验结果。为了测试不同的沃尔巴克氏菌感染在野外部署的适宜性，有必要将蚊子保持在受控的实验室环境中，跨越多代。我们描述了一种维护Ae的简单协议。实验室中的埃及伊蚊，无论是沃尔巴克氏菌感染的蚊子还是野生型蚊子都适用。这些方法最大限度地减少了实验室适应，并实施了异种杂交，以增加实验与野外蚊子的相关性。此外，菌落保持在最佳条件下，以最大限度地适应野外放飞。
Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, chikungunya and Zika. Wolbachia-infected mosquitoes can be released into the field to either reduce population sizes through incompatible matings or to transform populations with mosquitoes that are refractory to virus transmission. For these strategies to succeed, the mosquitoes released into the field from the laboratory must be competitive with native mosquitoes. However, maintaining mosquitoes in the laboratory can result in inbreeding, genetic drift and laboratory adaptation which can reduce their fitness in the field and may confound the results of experiments. To test the suitability of different Wolbachia infections for deployment in the field, it is necessary to maintain mosquitoes in a controlled laboratory environment across multiple generations. We describe a simple protocol for maintaining Ae. aegypti mosquitoes in the laboratory, which is suitable for both Wolbachia-infected and wild-type mosquitoes. The methods minimize laboratory adaptation and implement outcrossing to increase the relevance of experiments to field mosquitoes. Additionally, colonies are maintained under optimal conditions to maximize their fitness for open field releases.