Diosgenin and phytosterols content in five callus lines of Dioscorea balcanica
Diosgenin and phytosterols content in five callus lines of Dioscorea balcanica
复制标题
DOI：
10.1016/s0168-9452(98)00036-3
复制
发表时间：
1998-06-22
期刊：
影响因子：
5.2
通讯作者：
Ristic, M
中科院分区：
文献类型：
--
作者：
Savikin-Fodulovic, K;Grubisic, D;Ristic, M
薯蓣皂苷元是一种甾体化合物，作为甾体激素的天然来源，在制药工业中非常有用。它存在于许多野生的马桑属物种中，也存在于特有物种马桑属中。愈伤组织系从一个野生的波罗的海栽培品种，不同的薯蓣皂苷元，以及，在植物甾醇含量。通过光、暗、添加薯蓣皂苷元生物合成前体（胆固醇）和类胡萝卜素生物合成抑制剂（氟草松）等因素，筛选出薯蓣皂苷元含量高的愈伤组织系。光/暗条件影响愈伤组织系中薯蓣皂苷元的量，而植物甾醇的量的数量差异不太明显。此外，在光照下和黑暗中生长的愈伤组织的数量存在质的差异。在培养基中添加胆固醇和氟草酮增加了薯蓣皂苷元和植物甾醇的含量。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Diosgenin is a steroidal compound very useful in pharmaceutical industry as a natural source of steroidal hormones. It is found in many wild Dioscorea species and also in endemic species Dioscorea balcanica. Callus lines obtained from a wild Dioscorea balcanica cultivar, differed in diosgenin, as well as, in phytosterols content. An attempt to select callus lines with high levels of diosgenin considered a few factors such as light, dark, addition of precursor in diosgenin biosynthesis (cholesterol) and of inhibitor of carotenoid biosynthesis (norflurazon). Light/dark conditions affected the amount of diosgenin in callus lines while quantitative differences in phytosterols' amount were less pronounced. Moreover, there are qualitative differences in their amount between callus grown on light and in the dark. The addition of cholesterol and norflurazon in cultivation medium increased the amount of both diosgenin and phytosterols. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.