Antibiotic-releasing porous polymethylmethacrylate/gelatin/antibiotic constructs for craniofacial tissue engineering.
Antibiotic-releasing porous polymethylmethacrylate/gelatin/antibiotic constructs for craniofacial tissue engineering.
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DOI：
10.1016/j.jconrel.2011.01.029
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发表时间：
2011-05-30
期刊：
影响因子：
--
通讯作者：
Mikos AG
中科院分区：
文献类型：
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作者：
Shi M;Kretlow JD;Spicer PP;Tabata Y;Demian N;Wong ME;Kasper FK;Mikos AG
在两阶段再生医学方法的初始步骤中，开发了一种可释放药物的多孔聚甲基丙烯酸甲酯（PMMA）结构，以维持骨间隙并为伤口部位预充，从而重建显著的骨或复合颅面组织缺损。多孔聚甲基丙烯酸甲酯（PMMA）结构，包括明胶微粒（GMP）的制造顺序组装的GMP，抗生素粘菌素，和临床使用的骨水泥配方的PMMA粉末和甲基丙烯酸甲酯液体。研究了具有不同明胶掺入和药物含量的PMMA/明胶/抗生素结构，以阐明材料组成与结构性质（孔隙率和药物释放动力学）之间的关系。PMMA/明胶/抗生素结构的孔隙率范围在7.6±1.8-38.4±1.4%之间，取决于掺入的明胶量和为明胶溶胀而添加的药物溶液。构建体在10或14天内释放粘菌素，每天的平均释放速率高于10 μg/ml。PMMA/明胶/抗生素结构的孔隙率和体外粘菌素释放动力学通过改变材料组成和制造参数来调整。本研究证明了明胶结合PMMA结构作为功能性间隙维持器的潜力，可促进组织愈合/覆盖并解决局部感染，从而使最终再生组织结构获得更好的长期成功。
An antibiotic-releasing porous polymethylmethacrylate (PMMA) construct was developed to maintain the bony space and prime the wound site in the initial step of a two-stage regenerative medicine approach toward reconstructing significant bony or composite craniofacial tissue defects. Porous polymethylmethacrylate (PMMA) constructs incorporating gelatin microparticles (GMPs) were fabricated by the sequential assembly of GMPs, the antibiotic colistin, and a clinically used bone cement formulation of PMMA powder and methylmethacrylate liquid. PMMA/gelatin/antibiotic constructs with varying gelatin incorporation and drug content were investigated to elucidate the relationship between material composition and construct properties (porosity and drug release kinetics). The porosity of PMMA/gelatin/antibiotic constructs ranged between 7.6±1.8–38.4±1.4% depending on the amount of gelatin incorporated and the drug solution added for gelatin swelling. The constructs released colistin over 10 or 14 days with an average release rate per day above 10 µg/ml. The porosity and in vitro colistin release kinetics of PMMA/gelatin/antibiotic constructs were tuned by varying the material composition and fabrication parameters. This study demonstrates the potential of gelatin-incorporating PMMA constructs as a functional space maintainer for both promoting tissue healing/coverage and addressing local infections, enabling better long-term success of the definitive regenerated tissue construct.