A sensitive fluorometric assay for amino acid oxidases.
A sensitive fluorometric assay for amino acid oxidases.
复制标题
氨基酸氧化酶的灵敏荧光测定。
DOI：
10.1016/0003-2697(68)90343-6
复制
发表时间：
1968
影响因子：
2.9
通讯作者：
D. Wellner
中科院分区：
文献类型：
--
作者：
L. Lichtenberg;D. Wellner
介绍了一种氨基酸氧化酶测定方法，可测定0.005 μg酶或0.5 nmol氨基酸的氧化。它涉及到过氧化氢在过氧化物酶催化下对东莨菪碱的偶联氧化和剩余东莨菪碱的荧光测定。用这种方法证明了甘氨酸是l-氨基酸氧化酶的底物。
An assay for amino acid oxidases is described which is capable of measuring 0.005 μg of enzyme or the oxidation of 0.5 nmole of amino acid. It involves the coupled oxidation of scopoletin by hydrogen peroxide catalyzed by peroxidase and the fluorometric determination of the scopoletin remaining. By the use of this method, it is shown that glycine is a substrate of l-amino acid oxidase.