Bacterial community compositions of tomato (Lycopersicum esculentum Mill.) seeds and plant growth promoting activity of ACC deaminase producing Bacillus subtilis (HYT-12-1) on tomato seedlings
Bacterial community compositions of tomato (Lycopersicum esculentum Mill.) seeds and plant growth promoting activity of ACC deaminase producing Bacillus subtilis (HYT-12-1) on tomato seedlings
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DOI：
10.1007/s11274-013-1486-y
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发表时间：
2014-02
影响因子：
4.1
通讯作者：
Mingshuang Xu;J. Sheng;Lin Chen;Yejun Men;L. Gan;Shuntang Guo;Lin Shen
中科院分区：
文献类型：
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作者：
Mingshuang Xu;J. Sheng;Lin Chen;Yejun Men;L. Gan;Shuntang Guo;Lin Shen
植物种子内生细菌具有遗传性和多样性，因此对其进行研究具有重要意义。采用16 S rRNA基因PCR-RFLP（restriction fragment length polymorphism）技术，对4个番茄品种种子中芽孢杆菌的群落结构进行了研究。16 S rRNA基因系统发育分析表明，22个代表菌株分属于芽孢杆菌属（Bacillus）的5个种，且番茄品种间细菌组成存在显著差异。分离物表现出多种植物生长促进（PGP）性状：吲哚-3-乙酸产生37%;磷酸盐溶解37%;铁载体产生24%;潜在固氮85%和1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性6%。菌株HYT-12-1的ACC脱氨酶活性最高（112.02 nmolα-酮丁酸mg− 1蛋白h−1）。16 S rRNA基因序列分析表明，分离物HYT-12-1与B.枯草芽孢杆菌在非生物条件和温室条件下的PGP试验表明，菌株HYT-12-1具有促进番茄幼苗生长的能力。这是第一个描述番茄种子内内生芽孢杆菌群落的研究，结果表明B。菌株HYT-12-1作为生物肥料具有很大的工业应用潜力。
Study of endophytic bacteria within plant seeds is very essential and meaningful on account of their heritability and versatility. This study investigatedBacillusbacterial communities within the seeds of four commercial tomato varieties, by 16S rRNA gene PCR-RFLP (restriction fragment length polymorphism). Phylogenetic analysis of 16S rRNA gene sequences indicated that the 22 representative isolates belonged to five species of genusBacillusand the bacterial compositions showed remarkable differences among tomato varieties. Isolates exhibited multiple plant growth promoting (PGP) traits: 37 % of indole-3-acetic acid production; 37 % of phosphate solubilization; 24 % of siderophores production; 85 % of potential nitrogen fixation and 6 % of 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. Isolate HYT-12-1 was shown to have highest ACC deaminase activity (112.02 nmolα-ketobutyrate mg−1protein h−1) among the five ACC deamiase producing strains. 16S rRNA gene sequencing indicated that the isolate HYT-12-1 shared the highest sequence similarity (100 %) withB. subtilis. PGP experiments under gnotobiotic and greenhouse conditions revealed the ability of strain HYT-12-1 to enhance the growth of tomato seedlings. This is the first study to describe endophyticBacilluscommunities within tomato seeds, and the results suggest thatB. subtilisstrain HYT-12-1 would have a great potential for industrial application as biofertilizer in the future.