OXIDATION OF PALMITIC ACID BY MYCOBACTERIUM-LEPRAE IN AN AXENIC MEDIUM
OXIDATION OF PALMITIC ACID BY MYCOBACTERIUM-LEPRAE IN AN AXENIC MEDIUM
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DOI：
10.1128/jcm.26.1.18-21.1988
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发表时间：
1988-01-01
影响因子：
9.4
通讯作者：
FRANZBLAU, SG
中科院分区：
文献类型：
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作者：
FRANZBLAU, SG
研究了麻风分枝杆菌在无菌培养基中孵育期间氧化棕榈酸的能力。通过Buddemeyer型检测系统，部分纯化的裸鼠来源的M。发现麻风以线性方式从14 C标记的棕榈酸产生14 CO2至少1周。已知去除残留宿主组织的程序并没有降低14 CO2的释放速率，这表明正在测量细菌代谢。棕榈酸氧化是温度敏感的，具有明显的最佳温度为33 ℃。C，但pH不敏感。暴露于各种抗麻风药物1或2周的杆菌显示出显着降低的14 CO2演变率后，随后加入14 C-标记的棕榈酸。这种活性可以很容易地检测到106杆菌，从而表明其在临床药敏试验中使用的潜力。
The ability of Mycobacterium leprae to oxidize palmitic acid during incubation in an axenic medium was studied. By using a Buddemeyer-type detection system, partially purified nude-mouse-derived M. leprae was found to produce 14CO2 from 14C-labeled palmitic acid in a linear fashion for at least 1 week. Procedures known to remove residual host tissue did not diminish the rate of 14CO2 evolution, indicating that bacterial metabolism was being measured. Palmitate oxidation was temperature sensitive, with an apparent optimum of 33.degree. C, but pH insensitive. Bacilli exposed to a variety of antileprosy drugs for 1 or 2 weeks displayed significantly reduced rates of 14CO2 evolution upon subsequent addition of 14C-labeled palmitic acid. This activity could be readily detected with 106 bacilli, thus indicating its potential for use in clinical susceptibility testing.