Top-Down approach to nanotechnology for cell-on-chip applications.
Top-Down approach to nanotechnology for cell-on-chip applications.
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发表时间：
2010-08
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通讯作者：
A. Ninno;A. Gerardino;B. Girarda;G. Grenci;L. Businaro
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作者：
A. Ninno;A. Gerardino;B. Girarda;G. Grenci;L. Businaro
标准的细胞培养技术在几乎一个世纪里基本保持不变;今天，由于微米和纳米技术相关技术的进步，生物学家可用的工具箱正在迅速增长，使他们能够将实验能力提高到操纵单细胞和控制细胞环境的水平。从宏观尺度到微观尺度的转变为产生无法在培养皿和孔板中捕获的梯度和图案提供了前所未有的可能性。微米和纳米纤维将微图案化技术与先进的表面化学相结合，使其能够以细胞分辨率可重复地定制细胞微环境。研究具有用于可溶性因子信号传递的微流体网络的细胞外基质的纳米图案化表面具有调节空间和时间分化的细胞表型的潜力。此外，适当设计的微米和纳米粒子允许从空间和光谱的角度来看检测技术的极大增强。未来的前景将导致在单个芯片上开发不同群体的共培养平台，以模拟体内的细胞通信系统。在本文中，我们探讨了减少细胞环境的观察规模的重要性，并提供了一个基本的概述，微和纳米纤维的潜力和能力，这是今天可用的。
Standard cell culture techniques have remained basically unchanged for almost a century; today, thanks to advances in micro and nanotechnology related techniques, the toolbox available to biologists is rapidly growing, enabling them to increase their experimental capabilities up to the level of manipulating single cells and controlling cells environment. The transition from macro to microscale gives unprecedented possibilities to generate gradients and patterns that cannot be captured in Petri dishes and well plates. Micro and nanofabrication integrating micropatterning techniques with advanced surface chemistry makes it possible to reproducibly tailor the cell microenvironment at cellular resolution. Nanopatterned surfaces that investigate the extracellular matrix with microfluidic networks for soluble factor signaling delivery have the potential to modulate spatially and temporally differentiated cellular phenotypes. Moreover, properly engineered micro and nanopatterns allow a great enhancement in the detection techniques both from the spatial and spectroscopic point of view. Future perspective will lead to develop platforms for cocolture of different populations on a single chip, in order to mimic cellular communication systems in vivo. In this paper we explore the importance of reducing the observation scale of the cellular environment and provide an essential overview of the micro and nanofabrication potentials and capabilities which are today available.