Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya.
Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya.
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DOI：
10.1186/s13071-015-1106-9
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发表时间：
2015-09-30
影响因子：
3.2
通讯作者：
Xuan X
中科院分区：
文献类型：
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作者：
Adjou Moumouni PF;Aboge GO;Terkawi MA;Masatani T;Cao S;Kamyingkird K;Jirapattharasate C;Zhou M;Wang G;Liu M;Iguchi A;Vudriko P;Ybanez AP;Inokuma H;Shirafuji-Umemiya R;Suzuki H;Xuan X
牛巴贝虫、二联巴贝虫、泰勒虫属和边缘无形体感染在肯尼亚流行，但缺乏有关其基因型的足够信息。这项研究确定了感染肯尼亚牛的上述蜱传血寄生虫的遗传多样性。采用巢式 PCR 和测序技术确定了从 Ngong 和 Machakos 农场采集的 192 份牛血液样本中上述寄生虫的流行率和遗传多样性。 B. 牛球形体蛋白 4、B. bigemina 棒状体相关蛋白 1a、A. margine 主要表面蛋白 5、泰勒虫属 (Theileria spp)。使用18S rRNA、T. parva p104 和T. orientalis 主要梨质表面蛋白作为标记基因。 B. bovis、B. bigemina、T. parva、T. velifera、T. taurotragi、T. mutans 和 A. margine 在两个农场中都很常见，而 T. ovis、泰勒虫属 (Theileria sp.) 也很常见。 （水牛）和 T. orientalis 仅在 Ngong 农场发现。两个农场超过 50% 的阳性样本中都观察到了双重感染。巴贝虫寄生虫和边缘巴贝虫序列高度保守，而小巴贝虫和东方巴贝虫则具有多态性。马查科斯农场检测到来自牛的细小蜱虫。然而，在恩贡农场检测到牛和水牛来源的泰勒虫，表明牛和野生水牛之间存在相互作用。一般来说，肯尼亚检测到的病原体与其他非洲分离株有遗传相关性，但与其他大陆的分离株不同。目前的研究结果再次证实了蜱传血寄生虫在牛身上的流行和共同感染，以及野生动物在肯尼亚病原体传播和群体遗传学中的作用。本文的在线版本 (doi:10.1186/s13071-015-1106-9) 包含补充材料，可供授权用户使用。
Infections with Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale are endemic in Kenya yet there is a lack of adequate information on their genotypes. This study established the genetic diversities of the above tick-borne hemoparasites infecting cattle in Kenya. Nested PCR and sequencing were used to determine the prevalence and genetic diversity of the above parasites in 192 cattle blood samples collected from Ngong and Machakos farms. B. bovis spherical body protein 4, B. bigemina rhoptry-associated protein 1a, A. marginale major surface protein 5, Theileria spp. 18S rRNA, T. parva p104 and T. orientalis major piroplasm surface protein were used as the marker genes. B. bovis, B. bigemina, T. parva, T. velifera, T. taurotragi, T. mutans and A. marginale were prevalent in both farms, whereas T. ovis, Theileria sp. (buffalo) and T. orientalis were found only in Ngong farm. Co-infections were observed in more than 50 % of positive samples in both farms. Babesia parasites and A. marginale sequences were highly conserved while T. parva and T. orientalis were polymorphic. Cattle-derived T. parva was detected in Machakos farm. However, cattle and buffalo–derived Theileria were detected in Ngong farm suggesting interactions between cattle and wild buffaloes. Generally, the pathogens detected in Kenya were genetically related to the other African isolates but different from the isolates in other continents. The current findings reaffirm the endemicity and co-infection of cattle with tick-borne hemoparasites, and the role of wildlife in pathogens transmission and population genetics in Kenya. The online version of this article (doi:10.1186/s13071-015-1106-9) contains supplementary material, which is available to authorized users.