siRNAs can function as miRNAs
siRNAs can function as miRNAs
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DOI：
10.1101/gad.1064703
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发表时间：
2003-02-15
影响因子：
10.5
通讯作者：
Sharp, PA
中科院分区：
文献类型：
--
作者：
Doench, JG;Petersen, CP;Sharp, PA
随着RNA干扰（RNAi）及其相关现象的发现，RNA的新的调控作用不断出现。在这里，我们表明，在哺乳动物组织培养中，短干扰RNA（siRNA）可以抑制目标mRNA的表达，其3'UTR中具有部分互补的结合位点，很像内源性编码的microRNA（miRNA）的功能。这种抑制的机制是协同的，不同于siRNA切割mRNA的催化机制。使用siRNA研究翻译抑制有望剖析序列和结构决定因素以及miRNA抑制基因的一般机制。
With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA (siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3' UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs.