Antioxidative and anti-inflammatory effects of four cysteine-containing agents in striatum of MPTP-treated mice
Antioxidative and anti-inflammatory effects of four cysteine-containing agents in striatum of MPTP-treated mice
复制标题
DOI：
10.1016/j.nut.2007.05.004
复制
发表时间：
2007-07-01
期刊：
影响因子：
4.4
通讯作者：
Liu, Ting-Chun
中科院分区：
文献类型：
--
作者：
Chen, Chiu-Mei;Yin, Mei-Chin;Liu, Ting-Chun
目的：采用1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）染毒小鼠，观察N-乙酰半胱氨酸（NAC）、S-乙基半胱氨酸（SEC）、S-甲基半胱氨酸（SMC）和S-丙基半胱氨酸（SPC）对MPTP所致神经元损伤的保护作用。通过皮下注射MPTP（24 mg/kg体重）连续6天处理小鼠。结果：MPTP可显著降低纹状体谷胱甘肽含量，降低谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，升高丙二醛（MDA）水平，升高白细胞介素6（IL 6）和肿瘤坏死因子α（TNF α）水平（P < 0.05）。预先摄入NAC、SEC、SMC和SPC可显著减轻MPTP诱导的谷胱甘肽丢失，保留GPX和SOD活性，减轻氧化应激，并抑制MPTP诱导的IL-6和TNF-α升高（P < 0.05）。MPTP处理显著抑制GPX mRNA表达，增强TNF-a mRNA表达（P < 0.05）。与MPTP单独处理组相比，NAC、SEC、SMC和SPC预处理组GPX mRNA表达显著升高，TNF-α mRNA表达显著降低（P < 0.05），其中SPC对MPTP诱导的TNF-α mRNA表达的抑制作用最强（P < 0.05）。MPTP能显著降低纹状体多巴胺和3，4-二羟基苯乙酸含量（P < 0.05）。结论：含半胱氨酸化合物对帕金森病大鼠纹状体具有抗氧化和抗炎保护作用，可明显改善MPTP引起的多巴胺耗竭，增加多巴胺/3，4-二羟基苯乙酸含量（P < 0.05）。(c)2007爱思唯尔公司版权所有© 2016
Objectives: Mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were used to examine the neuroprotective effects of n-acetyl cysteine (NAC), s-ethyl cysteine (SEC), s-methyl cysteine (SMC), and s-propyl cysteine (SPC).Methods: Each agent at 1 g/L was directly added to the drinking water for 3 wk. Mice were treated by subcutaneous injection of MPTP (24 mg/kg body weight) for 6 consecutive days. The brain from each mouse was quickly removed and the striatum was collected for analyses.Results: The MPTP treatment significantly depleted striatal glutathione content, reduced the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase, increased malondialdehyde level, and elevated interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) levels in striatum (P < 0.05). The pre-intake of NAC, SEC, SMC, and SPC significantly attenuated MPTP-induced glutathione loss, retained the activity of GPX and SOD, diminished oxidative stress, and suppressed MPTP-induced elevation of IL-6 and TNF-alpha (P. < 0.05). MPTP treatment significantly suppressed GPX mRNA expression and enhanced TNF-a mRNA expression (P < 0.05). Compared with MPTP treatment alone, the pre-intake of NAC, SEC, SMC, and SPC significantly elevated GPX mRNA expression and diminished TNF-a mRNA expression (P < 0.05), in which SPC showed the greatest suppressive effect against MPTP-induced TNF-a mRNA expression (P < 0.05). Dopamine and 3,4-dihydroxyphenylacetic acid contents in the striatum were significantly decreased by MPTP treatment (P < 0.05). The pye-intake of four test agents significantly improved MPTP-induced dopamine depletion and increased dopamine/3,4-dihydroxyphenylacetic acid content (P < 0.05).Conclusion: These results suggest that these cysteine-containing compounds could provide anti-oxidative and anti-inflammatory protection for the striatum against the development of Parkinson's disease. (c) 2007 Elsevier Inc. All rights reserved..