Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors
Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors
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DOI：
10.1093/emboj/18.17.4689
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发表时间：
1999-09-01
期刊：
影响因子：
11.4
通讯作者：
Somssich, IE
中科院分区：
文献类型：
--
作者：
Eulgem, T;Rushton, PJ;Somssich, IE
欧芹WRKY蛋白包括植物特异性锌指型因子家族，其参与调节与病原体防御相关的基因。在体外，这些蛋白质特异性结合到功能上定义的含TGAC的Mi盒启动子元件内的致病相关的10类（PR-10）基因。在这里，我们提出了在体内的数据表明，WRKY 1是一种转录激活因子介导的真菌诱导子诱导的基因表达结合W盒元件。原位RNA杂交显示，WRKY 1基因是快速和局部激活的欧芹叶组织周围的真菌感染部位。欧芹原生质体中的瞬时表达研究搁置了WRKY 1启动子本身中的W盒元件的特定排列对于早期激活是必要的和足够的，并且WRKY 1结合到这些元件。我们的研究结果表明，WRKY转录因子在调控早期防御反应基因，包括WRKY 1的调控中发挥重要作用。
Parsley WRKY proteins comprise a family of plant-specific zinc-finger-type factors implicated in the regulation of genes associated with pathogen defence. In vitro, these proteins bind specifically to functionally defined TGAC-containing Mi box promoter elements within the Pathogenesis-Related Class 10 (PR-10) genes. Here we present in vivo data demonstrating that WRKY1 is a transcriptional activator mediating fungal elicitor-induced gene expression by binding to W box elements. In situ RNA hybridization revealed that the WRKY1 gene is rapidly and locally activated in parsley leaf tissue around fungal infection sites. Transient expression studies in parsley protoplasts shelved that a specific arrangement of W box elements in the WRKY1 promoter itself is necessary and sufficient for early activation and that WRKY1 binds to such elements. Our results demonstrate that WRKY transcription factors play an important role in the regulation of early defence-response genes including regulation of WRKY1.