Human erythrocyte membrane proteins of zone 4.5 exist as families of related proteins.
Human erythrocyte membrane proteins of zone 4.5 exist as families of related proteins.
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4.5 区的人类红细胞膜蛋白作为相关蛋白家族而存在。
DOI：
10.1152/ajpcell.1985.248.1.c70
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Goodman,SR
中科院分区：
文献类型：
--
作者：
Whitfield,CF;Coleman,DB;Kay,MM;Shiffer,KA;Miller,J;Goodman,SR
用免疫放射自显影和二维肽图技术分析了人红细胞膜4.5区的多肽组成。这些研究的结果表明，考马斯亮蓝图谱是恒定的，存在14个分离良好的条带。区带4.5多肽以至少四个家族的形式存在，其中两个或更多个组分具有密切相关的多肽主链。这些家族可以根据它们的提取特征、免疫交叉反应性和二维肽图来区分。一个家族与蛋白4.1相关，一个家族与带3相关，两个家族是独立的，与其他较大的膜蛋白不相似。数据显示，区域4.5中的所有可视化条带不具有相同的蛋白质组成，并且存在一些多肽的几种密切相关的形式。
An analysis of the polypeptide composition of zone 4.5 of human erythrocyte membranes has been done by immunoautoradiographic and two-dimensional peptide mapping techniques. Results of these studies demonstrated that the Coomassie blue profile was constant, with 14 well-resolved bands present. Zone 4.5 polypeptides existed as at least four families of two or more components with closely related polypeptide backbones. The families could be distinguished on the basis of their extraction characteristics, immunological cross-reactivity, and two-dimensional peptide maps. One family was related to protein 4.1, one family was related to band 3, and two families were independent and not similar to other larger membrane proteins. The data show that all of the visualized bands in zone 4.5 do not have the same protein composition and that several closely related forms of some polypeptides are present.