Structural analyses of sugar chains from Abrus precatorius agglutinin
Structural analyses of sugar chains from Abrus precatorius agglutinin
复制标题
相思子凝集素糖链的结构分析
DOI：
10.1016/0304-4165(88)90139-0
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
G. Funatsu
中科院分区：
文献类型：
--
作者：
Y. Kimura;S. Hase;T. Ikenaka;G. Funatsu
测定了鸡骨草凝集素天冬酰胺连接糖链的结构。糖链通过肼解作用从凝集素中释放出来。经N-乙酰化后，糖链的还原端残基与2-氨基吡啶偶联。通过凝胶过滤和HPLC纯化由此获得的吡啶基氨基（PA-）衍生物。纯化的衍生物的结构进行了鉴定，通过组分分析，比较其在HPLC上的洗脱位置与已知结构的PA-糖链，逐步外切糖苷酶消化，和部分乙酰解。从A.前体凝集素为Manα1 → 2 Man α1 → 6（Manα1 → 3）Manβ1 → 6（Manα1 → 2Manα2 → 2Manα1 → 3）Manα1 → GlcNAcβ1 → GlcNAc-PA，Manα1 → 2Manα1 → 6（Manα1 → 3）Manα1 → 6（Manα1 → 2Manα1 →3）Manβ1 → GlcNAcβ1 → GlcNAc-PA，Manα1 → 6（Manα1 → 3）Manα1 → 6（Manα1 → 2Manα1 → 3）Manβ1 → 4GlcNAcβ1 → 4GlcNAc-PA和Manα1 → 6（Manα1 → 34）（Xylβ1 → 2）Manβ1 → 4GlcNAcβ1 → 4（Fucα1 → 3）GlcNAc-PA。
The structures of asparagine-linked sugar chains ofAbrus precatoriusagglutinin were determined. The sugar chains were liberated from the lectin by hydrazinolysis. AfterN-acetylation, the reducing-end residues of the sugar chains were coupled with 2-aminopyridine. The pyridylamino (PA-) derivatives thus obtained were purified by gel filtration and HPLC. The structures of the purified derivatives were identified by component analysis, comparison of their elution positions on HPLC with those of PA-sugar chains of known structures, stepwise exoglycosidase digestion, and partial acetolysis. The major four surgar chains obtained fromA. precatoriusagglutinin were Manα1 → 2Manα1 → 6(Manα1 → 3)Manβ1 → 6(Manα1 → 2Manα2 → 2Manα1 → 3)Manα1 → GlcNAcβ1 → GlcNAc-PA, Manα1 → 2Manα1 → 6(Manα1 → 3)Manα1 → 6(Manα1 → 2Manα1 →3)Manβ1 → GlcNAcβ1 → GlcNAc-PA, Manα1 → 6(Manα1 → 3)Manα1 → 6(Manα1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc-PA, and Manα1 → 6(Manα1 → 34) (Xylβ1 → 2)Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 3)GlcNAc-PA.