Peptide‐mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel
Peptide‐mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel
复制标题
肽介导的大环内酯类耐药性揭示了新生肽出口通道中可能存在的特异性相互作用
DOI：
10.1111/j.1365-2958.2004.04290.x
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发表时间：
2004
影响因子：
3.6
通讯作者：
A. Mankin
中科院分区：
文献类型：
--
作者：
V. Vimberg;L. Xiong;Marne Bailey;T. Tenson;A. Mankin
特异性短肽的表达可以使细胞对大环内酯类抗生素产生抗性。从随机五肽表达文库中选择赋予对结构上不同的大环内酯类包括竹桃霉素、阿奇霉素、氮杂红霉素、交沙霉素和酮内酯头孢霉素的抗性的肽。对整个抗性肽集合的分析允许根据它们的序列相似性和它们赋予的抗性类型将它们分为五个不同的组。观察到大环内酯类抗生素的结构与赋予抗性的肽的序列之间存在强相关性。这种相关性表明新生肽与大环内酯类抗生素和/或核糖体之间的序列特异性相互作用可以发生在核糖体出口通道中。
Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysis of the entire collection of the resistance peptides allowed their classification into five distinct groups according to their sequence similarity and the type of resistance they confer. A strong correlation was observed between the structures of macrolide antibiotics and sequences of the peptides conferring resistance. Such a correlation indicates that sequence‐specific interactions between the nascent peptide and the macrolide antibiotic and/or the ribosome can occur in the ribosomal exit tunnel.
影响因子：
5.7
作者：
Schlünzen, F;Harms, JM;Yonath, A
通讯作者：
Yonath, A
影响因子：
56.9
作者：
Nissen, P;Hansen, J;Steitz, TA
通讯作者：
Steitz, TA