Biogenesis of hepatocyte plasma-membrane domains. Incorporation of (3H)fucose into plasma-membrane and golgi-apparatus glycoproteins.
Biogenesis of hepatocyte plasma-membrane domains. Incorporation of (3H)fucose into plasma-membrane and golgi-apparatus glycoproteins.
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肝细胞质膜域的生物发生。
DOI：
10.1042/bj1920903
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
P. Vischer
中科院分区：
文献类型：
--
作者：
W. H. Evans;N. Flint;P. Vischer
1.给大鼠尾内注射[3H]岩藻糖，观察其掺入血清、高尔基体和质膜亚组分岩藻蛋白的速率，最多持续2h。2.掺入高尔基体和分泌囊泡组分达到最大值在15分钟或更短的时间，但大部分的放射性与分泌糖蛋白类。并入到正弦质膜馏分达到最大值在30分钟，符合岩藻糖蛋白的最大释放到血清中。贴壁细胞和小管质膜组分标记稍晚，并在较低的速率和比放射性。3.通过聚丙烯酰胺凝胶电泳分离的岩藻蛋白的荧光照相有助于区分主要的分泌型和膜结合型糖蛋白。结果表明，一个主要的生物发生顺序可能是从高尔基体到高尔基体分泌元件到窦状质膜。4.掺入的动力学使得糖蛋白不太可能快速和直接插入到胆管质膜中。提出了一种通过膜介导的细胞内途径将糖蛋白从血窦直接转移到胆管质膜的途径。
1. Rats were injected intracaudally with [3H]fucose and its rate of incorporation into the fucoproteins of serum, Golgi and plasma-membrane subfractions was followed for up tp 2h. 2. Incorporation into the Golgi dictyosome and secretory-vesicular fractions reached a maximum at 15 min or less, but most of the radioactivity was associated with classes of secretory glycoproteins. Incorporation into sinusoidal plasma-membrane fractions reached a maximum at 30 min, coinciding with the maximum release of fucoproteins into the serum. Contiguous and canalicular plasma-membrane fractions were labelled slightly later and at a lower rate and specific radioactivity. 3. Fluorography of fucoproteins separated by polyacrylamide-gel electrophoresis helped to distinguish between the major secretory and membrane-bound glycoproteins. The results show that a major biogenetic sequence is probably from Golgi dictyosomes to Golgi secretory elements to a sinusoidal plasma membrane. 4. The kinetics of incorporation make it unlikely that there is rapid and direct insertion of glycoproteins into the bile-canalicular plasma membrane. A route involving direct transfer of glycoproteins via a membrane-mediated intracellular path from the blood sinusoidal to the bile-canalicular plasma membranes is proposed.