Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection
Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection
复制标题
DOI：
10.1111/j.1365-2672.2004.02283.x
复制
发表时间：
2004-01-01
影响因子：
4
通讯作者：
Makino, SI
中科院分区：
文献类型：
--
作者：
Cheun, HI;Kawamoto, K;Makino, SI
目的：为建立一种经济、安全、简便的猪丹毒疫苗接种体系，将猪丹毒丝菌spaA基因插入穿梭质粒pSECE 1中，构建了pSECE1.3。L.乳酸菌在生长中保持稳定的抗原性而不降解。小鼠经口和鼻内接种携带pSECE1.3的L.结果表明，小鼠经100 LD_（50）E. rhusiopathiae Tama-96在大腿内侧的感染。研究的意义和影响：在这种疫苗接种系统中，不需要纯化抗原和注射，从而降低了生产成本，减少了劳动力，减轻了动物的压力。该乳酸菌疫苗接种系统应是安全且适用于多价疫苗的载体。
Aims: To develop an economical, safe and simple vaccination system against swine erysipelas using SpaA-antigen producing Lactococcus lactis.Methods and Results: The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1.3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1.3-carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD50 of E. rhusiopathiae Tama-96 in the inner thigh.Conclusions: SpaA-producing L. lactis appears useful as an effective subunit vaccine against swine erysipelas.Significance and Impact of the Study: In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.