Constant-volume hydrogel osmometer: A new device concept for miniature biosensors
Constant-volume hydrogel osmometer: A new device concept for miniature biosensors
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DOI：
10.1021/bm0255894
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发表时间：
2002-11-01
影响因子：
6.2
通讯作者：
Magda, JJ
中科院分区：
文献类型：
--
作者：
Han, IS;Han, MH;Magda, JJ
提出了一种新型的生物传感器，它结合了“智能”水凝胶的识别特性和微型压力传感器的灵敏度和可靠性。在所提出的设备中，分析物引起的环境响应性水凝胶的渗透膨胀压力的变化通过将其限制在刚性半透膜和微型压力传感器的隔膜之间的小的可植入外壳内来测量。使用pH敏感性水凝胶在体外进行该器械的原理验证试验，将相同水凝胶的渗透性去溶胀数据用作比较基准。水凝胶的溶胀压力是准确地确定从对水库的已知渗透应力的渗透去溶胀测量。膨胀压力与盐浓度的值与初步版本的传感器测量同意渗透消胀的结果。通过用各种酶或侧基结合部分修饰水凝胶，传感器具有以良好的特异性检测宽范围的生物分析物的潜力。
A new type of biosensor is proposed that combines the recognition properties of "intelligent" hydrogels with the sensitivity and reliability of microfabricated pressure transducers. In the proposed device, analyte-induced changes in the osmotic swelling pressure of an environmentally responsive hydrogel are measured by confining it within a small implantable enclosure between a rigid semipermeable membrane and the diaphragm of a, miniature pressure transducer. Proof-of-principle tests of this device were performed in vitro using pH-sensitive hydrogels, with osmotic deswelling data for the same hydrogels used as a benchmark for comparison. The swelling pressure of the hydrogel was accurately determined from osmotic deswelling measurements against reservoirs of known osmotic stress. Values of swelling pressure vs salt concentration measured with a preliminary version of the sensor agree well with osmotic deswelling results. Through modification of the hydrogel with various enzymes or pendant binding moieties, the sensor has the potential to detect a wide range of biological analytes with good specificity.