Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experimental animals
Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experimental animals
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DOI：
10.1016/j.bbabio.2007.10.014
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Shinohara, Yasuo
中科院分区：
文献类型：
--
作者：
Watanabe, Masahiro;Yamamoto, Takenori;Shinohara, Yasuo
为了鉴定在冷暴露大鼠中棕色脂肪组织 (BAT) 中表达上调或下调的基因，我们对从冷暴露大鼠和室温下保存的大鼠的 BAT 制备的 RNA 样本进行了微阵列分析。先前报道的冷暴露大鼠 BAT 中解偶联蛋白 (UCP1)、II 型碘甲状腺氨酸脱碘酶 (DIO2) 和 III 型腺苷酸环化酶 (AC3) 的转录水平高于室温大鼠的转录水平，这一结果得到证实。除了这些变化之外，在冷暴露大鼠的 BAT 中还检测到一些似乎与细胞周期调节和 DNA 复制过程相关的基因转录水平显着升高，这可能反映了棕色脂肪细胞对冷暴露的反应显着增殖。通过微阵列分析也检测到冷暴露大鼠的BAT中编码肌节线粒体型肌酸激酶的基因表达上调，但随后的Northern分析显示，在冷暴露大鼠的BAT中，不仅肌节线粒体型肌酸激酶的表达升高，而且其他2种亚型（即细胞质脑型和细胞质肌型）的表达也升高。微阵列分析还揭示了肌红蛋白在 BAT 中的显着表达及其在冷暴露大鼠 BAT 中的升高，这一结果得到了校准 Northern 分析的支持。相反，在冷暴露大鼠的 BAT 中，G 蛋白信号调节因子 2 和 IMP 脱氢酶 I 等多个基因下调。讨论了这些伴随寒冷暴露的变化的生理意义。 (C) 2007 年由 Elsevier B.V. 出版
To identify genes whose expression in brown adipose tissue (BAT) is up- or down-regulated in cold-exposed rats, we performed microarray analysis of RNA samples prepared from the BAT of cold-exposed rats and of rats kept at room temperature. Previously reported elevations of transcript levels of uncoupling protein (UCP1), type II iodothyronine deiodinase (DIO2), and type III adenylate cyclase (AC3) in the BAT of cold-exposed rats over those in that of rats maintained at room temperature were confirmed. In addition to these changes, remarkable elevations of the transcript levels of several genes that seemed to be associated with the processes of cell-cycle regulation and DNA replication were detected in the BAT of cold-exposed rats, possibly reflecting the significant proliferation of brown adipocytes in response to cold exposure. Up-regulation of the gene encoding sarcomeric mitochondrial type creatine kinase in the BAT of cold-exposed rats was also detected by microarray analysis, but subsequent Northern analysis revealed that the expression of not only the sarcomeric mitochondrial type enzyme, but also that of 2 other subtypes, i.e., cytoplasmic brain type and cytoplasmic muscle type, was elevated in the BAT of cold-exposed rats. Microarray analysis also revealed a significant expression of myoglobin in BAT and its elevation in the BAT of cold-exposed rats, and this result was supported by calibrated Northern analysis. On the contrary, several genes such as regulator of G-protein signaling 2 and IMP dehydrogenase I were down-regulated in the BAT of cold-exposed rats. The physiological meaning of these changes accompanying cold exposure was discussed. (C) 2007 Published by Elsevier B.V.