A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.
A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.
复制标题
一种新型手持式微流控细胞仪，通过分析受损细胞分布来评估辐照损伤
DOI：
10.1038/srep23165
复制
发表时间：
2016-03-17
影响因子：
4.6
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Wang J;Fan Z;Zhao Y;Song Y;Chu H;Song W;Song Y;Pan X;Sun Y;Li D
空间辐射对细胞的损伤是不均衡的。细胞损伤分布的检测在放射医学及相关研究中具有非常重要的作用。本文研制了一种新型手持式微流控流式细胞仪，用于评价细胞的辐射损伤程度。该仪器克服了商用流式细胞仪体积大、成本高的缺点，能够准确、快速地评估细胞的辐射损伤，具有现场应用的潜力。通过同时检测γ-H_2AX的免疫荧光强度和电阻脉冲传感器信号来分析辐射损伤细胞的分布。γ-H_2AX荧光强度提供了细胞内辐射损伤程度的信息。具有γ-H_2AX荧光信号的细胞数量与放射免疫分析检测到的细胞总数的比率表示受到辐射损伤的细胞的百分比。研制的手持式微流控流式细胞仪与商用共聚焦显微镜的对比实验表明，该仪器的检测性能一致，具有可比性。
Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propose overcomes the shortcomings (e.g., large volume and high cost) of commercial flow cytometers and can evaluate the radiation damage of cells accurately and quickly with potential for onsite applications. The distribution of radiation-damaged cells is analyzed by a simultaneous detection of immunofluorescence intensity of γ-H2AX and resistance pulse sensor (RPS) signal. The γ-H2AX fluorescence intensity provides information of the degree of radiation damage in cells. The ratio of the number of cells with γ-H2AX fluorescence signals to the total numbers of cells detected by RPS indicates the percentage of the cells that are damaged by radiation. The comparison experiment between the developed hand-held microfluidic flow cytometer and a commercial confocal microscope indicates a consistent and comparable detection performance.