Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.
Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.
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Lottiidibacillus patelloidae gen.
DOI：
10.1007/s10482-018-01213-z
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发表时间：
2019-05-01
影响因子：
2.6
通讯作者：
Shao, Zongze
中科院分区：
文献类型：
--
作者：
Liu, Renju;Huang, Zhaobin;Shao, Zongze
对采自厦门潮间带岩石中的一种海洋帽贝（Patellloida saccharinalanx [Reeve，1855]）的菌株SA 5d-4 T进行了分类学研究。菌株SA 5d-4 T为革兰氏阳性好氧菌，无鞭毛，不运动，丝状，菌落微黄色，不产孢。该菌株在28 °C、pH值7.0-8.0和1-2%（w/v）氯化钠存在下生长最佳。鉴定的主要细胞脂肪酸为iso-C15：0、iso-C17：0ω 10 c和iso-C17：0。主要呼吸醌是甲基萘醌-7。主要磷脂被鉴定为二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。基因组DNA G + C含量为35.3mol%，由基因组序列草图计算。基于16 S rRNA基因序列的系统发育分析表明，菌株SA 5d-4 T属于芽孢杆菌科中的一个新属，与Bacillus taeanensis BH 030017 T、Bacillus algicolaKMM 3737 T、Bacillus alkalinitrilicusANL-iso 4 T、Bacillus hwajinpoensisSW-72 T的同源性分别为95.6%、95.2%、95.1%、94.9%、94.9%。碱性重氮营养厌氧杆菌MS 6 T的耐药率为94.6%。全基因组系统发育分析表明，菌株SA 5d-4 T与B. taeanensisBH 030017 T.菌株SA 5d-4与B. taeanensisBH 030017 T为69.6%。根据多相分类学特征，菌株SA 5d-4 T代表一个新属的新种，命名为Lottiidibacilluspatelloidaegen。十一月，新种，模式菌株SA 5d-4 T（= MCCC 1A 11654 T = KCTC 33831 T）。系统发育分析表明，B. Taeanensis应转入一个新属，命名为Maribacillus，即Maribacillus taeanensiscomb。十一月，模式菌株BH 030017 T（= KCTC 3918 T = DSM 16466 T）。
A taxonomic study was carried out on strain SA5d-4T, which was isolated from a marine limpet (Patelloida saccharinalanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4Twas aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0–8.0, and in the presence of 1–2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C15:0, iso-C17:0ω10c, and iso-C17:0. The dominated respiratory quinone was menaquinone-7. The major phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C content was 35.3 mol%, calculated from a draft genome sequence. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that strain SA5d-4Tbelongs to a new genus within the familyBacillaceae, and this gene shares 95.6% similarity with that fromBacillus taeanensisBH030017T, 95.2% withBacillus algicolaKMM 3737T, 95.1% withBacillus alkalinitrilicusANL-iso4T, 94.9% withBacillus hwajinpoensisSW-72T, and 94.6% withAnaerobacillus alkalidiazotrophicusMS6T. Whole genome phylogenetic analyses indicated that strain SA5d-4Tformed a monophyletic branch withB. taeanensisBH030017T. The average nucleotide identity between strain SA5d-4TandB. taeanensisBH030017Twas 69.6%. Based on polyphasic taxonomic characteristics, strain SA5d-4Trepresents a novel species of a new genus, for which the nameLottiidibacillus patelloidaegen. nov., sp. nov., is proposed with the type strain SA5d-4T(= MCCC 1A11654T= KCTC 33831T). Based on phylogenetic analyses,B. taeanensisshould be transferred to a new genus, namedMaribacillus, asMaribacillus taeanensiscomb. nov., with type strain BH030017T(= KCTC 3918T= DSM 16466T).