Expression and anti-bacterial activity of human parotid secretory protein (PSP)
Expression and anti-bacterial activity of human parotid secretory protein (PSP)
复制标题
DOI：
10.1042/bst0310815
复制
发表时间：
2003-08-01
影响因子：
3.9
通讯作者：
Gorr, SU
中科院分区：
文献类型：
--
作者：
Geetha, C;Venkatesh, SG;Gorr, SU
腮腺分泌蛋白（Parotid Secretory Protein，PSP）是小鼠和大鼠腮腺中含量丰富的蛋白质。在人类20号染色体上发现了一个相关序列（C20 orf 70）。本研究的目的是确定PSP是否在人腮腺中表达。从人腮腺cDNA样品扩增人PSP的cDNA。一种肽抗体，提出了PSP的C-末端肽，确定了人腮腺组织中的蛋白免疫荧光显微镜。免疫亲和层析表明PSP在人唾液中有表达。PSP与杀菌/通透性增加蛋白（BPI）有关。为了测试PSP是否表现出抗菌活性，在大鼠GH4 C1细胞中表达表位标记的PSP。分泌培养基表现出抑菌或杀菌作用的铜绿假单胞菌在菌落形成试验相比，分泌培养基从GH4 C1细胞，不表达PSP。这些结果表明PSP在人腮腺和唾液中表达，在那里它作为BPI样抗菌蛋白发挥作用。
Parotid secretory protein (PSP) is an abundant protein in mouse and rat parotid glands. A related sequence (C20orf70) was identified on human chromosome 20. The goal of this study was to determine if PSP is expressed in the human parotid gland. The cDNA for human PSP was amplified from a human parotid cDNA sample. A peptide antibody, raised to the C-terminal peptide of PSP, identified the protein in human parotid tissue by immunofluorescence microscopy. Immunoaffinity chromatography suggested that PSP was expressed in human saliva. PSP is related to bactericidal/permeability-increasing protein (BPI). To test if PSP exhibits anti-bacterial activity, epitope-tagged PSP was expressed in rat GH4C1 cells. The secretion medium exhibited bacteristatic or bactericidal effects on Pseudomonas aeruginosa in a colony-forming assay when compared with secretion medium from GH4C1 cells that did not express PSP. These results suggest that PSP is expressed in the human parotid gland and saliva, where it functions as a BPI-like anti-bacterial protein.