Identification and characterization of a new coleviroid (CbVd-5)
Identification and characterization of a new coleviroid (CbVd-5)
复制标题
新型类病毒 (CbVd-5) 的鉴定和表征
DOI：
10.1007/s00705-008-0276-6
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发表时间：
2009-02-01
影响因子：
2.7
通讯作者：
Li, Shi-Fang
中科院分区：
文献类型：
--
作者：
Hou, Wan-Ying;Sano, Teruo;Li, Shi-Fang
从中国的肠道中检测到类病毒核糖核酸。它由274个核苷酸组成，与已知最近的类病毒物种的一个成员有66%的序列同源性。预测的二级结构为棒状，具有广泛的碱基配对，具有GenusColeviroid的保守区特征。还鉴定了在该属部分成员中高度保守的两个末端序列。类病毒RNA通过斜行接种成功地转移到结肠癌细胞中。该类病毒被鉴定为Coleviroid属的一个新成员，我们暂定将其命名为Coleus blumei viroid 5(CbVd-5)。
A viroid-like RNA was detected from coleus (Coleus blumei) in China. It consisted of 274 nucleotides and had 66% sequence identity with a member of the closest known viroid species. The predicted secondary structure is rod-shaped with extensive base pairing, and it has the conserved region characteristic of the genusColeviroid. Two terminal sequences that are highly conserved among some members of the genus were also identified. The viroid-like RNA was successfully transmitted to coleus by slash-inoculation. This viroid was identified as a new member of the genusColeviroid, and we tentatively propose the name Coleus blumei viroid 5 (CbVd-5).