FRET probes for measuring sphingolipid metabolizing enzyme activity
FRET probes for measuring sphingolipid metabolizing enzyme activity
复制标题
DOI：
10.1016/j.chemphyslip.2018.09.014
复制
发表时间：
2018-11-01
影响因子：
3.4
通讯作者：
Arenz, Christoph
中科院分区：
文献类型：
--
作者：
Mohamed, Zainelabdeen H.;Rhein, Cosima;Arenz, Christoph
Forster共振能量转移（FRET）探针是生物学中的独特工具，因为它们允许对活细胞内的生物分子或人工底物的某种状态进行真实的非破坏性监测。FRET底物指示它们的相对切割速率，从而指示给定酶的原位活性。与淬灭探针或开启探针相比，FRET探针的两个单独信号之一可以用作内部参考，这使得不依赖于细胞递送的切割事件的比率成像和定量成为可能。在这篇综述中，我们描述了鞘脂FRET探针的第一个例子相比，不同的替代探针。最后，我们展望了未来的探针及其潜在的应用。
Forster resonance energy transfer (FRET) probes are unique tools in biology, as they allow for a non-destructive monitoring of a certain state of a biomolecule or of an artificial substrate within living cells in real time. FRET substrates indicate their relative cleavage rate and thus the in situ activity of a given enzyme. In contrast to quenched probes or turn-on probes, one of the two separate signals of the FRET probes can be used as internal reference, which makes ratio-imaging and quantitation of cleavage events independent of cellular delivery possible. In this review, we describe the first examples of sphingolipid FRET probes in comparison to different alternative probes. Finally, we give an outlook on future probes and their potential application.