miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN
miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN
复制标题
外泌体转移的miR-93-5p通过靶向PTEN的细胞间通讯促进食管癌细胞的增殖
DOI：
10.3967/bes2018.023
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发表时间：
2018-03-01
影响因子：
3.5
通讯作者：
Liu Ran
中科院分区：
文献类型：
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作者：
Liu Meng Xin;Liao Juan;Liu Ran
目的探讨血浆miR-93-5p与食管癌发病风险的关系，以及miR-93-5p通过外泌体对食管癌细胞生物学功能的影响。方法采用实时荧光定量PCR方法分析食管癌患者和健康对照组血浆miR-93-5p的表达。采用条件logistic回归和生存分析分析miR-93-5p对食管癌发生风险及预后的影响。通过建立供细胞体外共培养模型，研究miR-93-5p对受体细胞生物学功能的影响。通过荧光素酶报告基因实验和Western Blotting验证miR-93-5p的靶基因。结果血浆miR-93-5p表达上调可显著增加食管癌的发生风险，并与预后不良相关。外泌体转运的miR-93-5p促进受体食管癌细胞的增殖，影响PTEN及其下游蛋白p21和cyclin Dl的表达。结论本研究为食管癌诊断和预后的生物标志物鉴定提供了参考。
Objective To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes.Methods The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.Results Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin Dl.Conclusion Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.