Rho mediates cytokinesis and epiboly via ROCK in zebrafish
Rho mediates cytokinesis and epiboly via ROCK in zebrafish
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DOI：
10.1002/mrd.20290
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Lee, SJ
中科院分区：
文献类型：
--
作者：
Lai, SL;Chang, CN;Lee, SJ
为了研究Rho对斑马鱼胚胎发育的调控，我们将肉毒梭菌C3-外切酶（C3）注射到斑马鱼胚胎中。我们发现，C3抑制胞质分裂在早期卵裂。C3抑制似乎是特异性的RhoA，因为组成型活性RhoA可以部分挽救C3诱导的缺陷。肌动蛋白和分裂沟相关β-连环蛋白的分布被C3破坏。Belbbistattin是一种肌球蛋白II抑制剂，也会导致卵裂球解体。这表明Rho通过卵裂沟蛋白组装和肌动球蛋白环收缩介导胞质分裂。此外，C3阻断细胞运动epilboly和原肠胚形成的损害没有尾巴和goosecoid表达的胚盘前亚军细胞和背唇的囊胚，分别证明。Rho相关激酶（ROK/ROCK）拮抗剂Y-27632对斑马鱼生长发育的抑制作用与C3相似。两者合计，这些结果表明，Rho介导的卵裂沟蛋白组装过程中的胞质分裂和细胞迁移过程中的外包和原肠胚通过一个ROK/ROCK依赖性途径。(c)2005 Wiley-Liss，Inc.
To study the regulation of embryonic development by Rho, we microinjected Clostridium botulinum C3-exoenzyme (C3) into zebrafish embryos. We found that C3 inhibited cytokinesis during early cleavages. C3 inhibition appeared to be specific on RhoA, since the constitutively active RhoA could partially rescued the C3-induced defects. Distributions of actin and the cleavage furrow associated beta-catenin were disrupted by C3. Belbbistatin, a myosin II inhibitor, also caused blastomeres disintegration. It suggested that Rho mediates cytokinesis via cleavage furrow protein assembly and actomyosin ring constriction. Furthermore, C3 blocked cellular movements during epilboly and gastrulation as evident by the impairment on no tail and goosecoid expression in blastoderm front runner cells and the dorsal lip of blastopore, respectively. Y-27632, an antagonist of Rho-associated kinase (ROK/ROCK), had the similar inhibitory effects on zebrafish development as the C3 treatments. Taken together, these results suggest that Rho mediates cleavage furrow protein assembly during cytokinesis and cellular migration during epiboly and gastrulation via a ROK/ROCK-dependent pathway. (c) 2005 Wiley-Liss, Inc.