Fluorescence diagnosis of metastatic lymph nodes using 5-aminolevulinic acid (5-ALA) in a mouse model of colon cancer
Fluorescence diagnosis of metastatic lymph nodes using 5-aminolevulinic acid (5-ALA) in a mouse model of colon cancer
复制标题
使用 5-氨基乙酰丙酸 (5-ALA) 对结肠癌小鼠模型中的转移淋巴结进行荧光诊断
DOI：
10.1016/j.jss.2011.10.031
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发表时间：
2012
期刊：
影响因子：
2.2
通讯作者：
Sakai Y
中科院分区：
文献类型：
--
作者：
Kato S;Kawamura J;Kawada K;Hasegawa S;Sakai Y
背景：淋巴结转移是影响结直肠癌患者预后的重要因素之一。虽然局部淋巴结需要手术切除和病理检查，但术中淋巴结转移的诊断技术仍有待完善。5-氨基乙酰丙酸（5-ALA）荧光诊断是一种很有前途的评估各种恶性肿瘤的技术。外源性给药5-ALA后，原卟啉IX （PPIX）在恶性细胞中积累，并可以红色荧光检测到。在这项研究中，我们研究了利用5-ALA荧光诊断检测结肠癌小鼠模型淋巴结转移的有效性。材料与方法用人结肠癌细胞系HCA7接种裸鼠盲肠壁制备原位结肠癌模型。3周后，腹腔（IP）或口服（PO）给予40 mg/kg的5-ALA。结果IP组转移淋巴结及其他恶性病变（包括原发肿瘤和腹腔植入物）均检测到PPIX荧光，而非转移淋巴结均为荧光阴性。而PO组癌组织未见明显荧光。结论腹腔注射5-ALA诱导的sppix荧光可准确诊断小鼠转移性淋巴结。这项技术可以促进术中结肠癌淋巴结转移的临床诊断。
BACKGROUNDLymph node metastasis is one of the most critical prognostic factors in patients with colorectal cancer. Although regional lymph nodes should be surgically resected and pathologically examined, techniques for the intraoperative diagnosis of lymph node metastasis remain to be well established. Fluorescence diagnosis using 5-aminolevulinic acid (5-ALA) is a promising technique for evaluating various malignancies. After exogenous administration of 5-ALA, protoporphyrin IX (PPIX) accumulates in malignant cells and can be detected as red fluorescence. In this study, we investigated the usefulness of fluorescence diagnosis using 5-ALA for the detection of lymph node metastasis in a mouse model of colon cancer.MATERIALS AND METHODSAn orthotopic colon cancer model was prepared by inoculating the cecal wall of nude mice with HCA7, a human colon adenocarcinoma cell line. After 3 wk, 40 mg/kg of 5-ALA was administered intraperitoneally (IP) or orally (PO). Fluorescence diagnosis with a D-Light System (Karl Storz) was then performed after 3 or 6 h.RESULTSIn the IP group, PPIX fluorescence was detected in metastatic lymph nodes as well as in other malignant lesions, including primary tumors and abdominal implantations, while non-metastatic nodes were fluorescence-negative. In contrast, no obvious fluorescence was detected in cancerous tissues in the PO group.CONCLUSIONSPPIX fluorescence induced by intraperitoneal injection of 5-ALA allows metastatic lymph nodes to be accurately diagnosed in this mouse model. This technique may facilitate the intraoperative diagnosis of lymph node metastases from colon cancer in a clinical setting.