PRGF exerts more potent proliferative and anti-inflammatory effects than autologous serum on a cell culture inflammatory model
PRGF exerts more potent proliferative and anti-inflammatory effects than autologous serum on a cell culture inflammatory model
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DOI：
10.1016/j.exer.2016.08.012
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发表时间：
2016-10-01
影响因子：
3.4
通讯作者：
Orive, G.
中科院分区：
文献类型：
--
作者：
Anitua, E.;Muruzabal, F.;Orive, G.
眼移植物抗宿主病（oGVHD）是全身炎症性疾病的一部分，通常影响眼表组织，表现为干眼综合征。目前的治疗效果并不令人满意。血液衍生产品，如富含生长因子 (PRGF) 的血浆，成为治疗这种疾病的潜在疗法。本研究的目的是评估 PRGF（一种自体富血小板血浆滴眼剂）与从 oGVHD 患者获得的自体血清 (AS) 相比，对在促炎环境中培养的眼表细胞的组织再生和抗炎能力。 PRGF 和 AS 取自四名 GVHD 患者。在促炎条件下培养并用 PRGF 或 AS 滴眼液治疗后的角膜和结膜成纤维细胞中测量细胞增殖和炎症标志物、细胞间粘附分子-1 (ICAM-1) 和环氧合酶-2 (COX-2)。此外，用 PRGF 和 AS 处理后，细胞增殖增加，但用 PRGF 处理角膜细胞时，这种增强作用明显更高。 PRGF 滴眼液显示，相对于初始炎症情况和 AS 治疗，炎症标志物均显着减少。我们的结果得出结论，对于用促炎性IL-1β和TNFα处理的眼表成纤维细胞，PRGF比自体血清发挥更有效的再生和抗炎作用。 (C) 2016 Elsevier Ltd. 保留所有权利。
Ocular graft versus host disease (oGVHD) is part of a systemic inflammatory disease that usually affects ocular surface tissues manifesting as a dry eye syndrome. Current treatments provide unsatisfactory results. Blood-derived products, like plasma rich in growth factors (PRGF) emerge as a potential therapy for this disease. The purpose of this study was to evaluate the tissue regeneration and anti-inflammatory capability of PRGF, an autologous platelet enriched plasma eye-drop, compared to autologous serum (AS) obtained from oGVHD patients on ocular surface cells cultured in a pro-inflammatory environment. PRGF and AS were obtained from four GVHD patients. Cell proliferation and inflammation markers, intercellular adhesion molecule-1 (ICAM-1) and cyclooxygenase-2 (COX-2), were measured in corneal and conjunctival fibroblastic cells cultured under pro-inflammatory conditions and after treatment with PRGF or AS eye drops. Moreover, cell proliferation increased after treatment with PRGF and AS, though this enhancement in the case of keratocytes was significantly higher with PRGF. PRGF eye drops showed a significant reduction of both inflammatory markers with respect to the initial inflammatory situation and to the AS treatment. Our results concluded that PRGF exerts more potent regenerative and anti-inflammatory effects than autologous serum on ocular surface fibroblasts treated with pro inflammatory IL-1 beta and TNF alpha. (C) 2016 Elsevier Ltd. All rights reserved.