Altered placental syncytin and its receptor ASCT2 expression in placental development and pre-eclampsia
Altered placental syncytin and its receptor ASCT2 expression in placental development and pre-eclampsia
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DOI：
10.1111/j.1471-0528.2005.00843.x
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发表时间：
2006-02-01
影响因子：
5.8
通讯作者：
Chen, H
中科院分区：
文献类型：
--
作者：
Chen, CP;Wang, KG;Chen, H
目的探讨与合体滋养层形成有关的融合膜蛋白合胞素的变化，及其受体ASCT 2在胎盘发育和先兆子痫中的表达。胎盘样本收集自一家三级医疗机构，16名患有先兆子痫的妇女和58名孕妇被诊断为妊娠（妊娠5-19周）选择性终止妊娠，早产，方法采用实时荧光定量聚合酶链反应（RT-PCR）技术，检测35例正常妊娠妇女胎盘组织中syncytin和ASCT 2的表达（范围从5至40周的妊娠）和先兆子痫胎盘（n=16）与孕龄匹配的对照组（n = 16）相比的变化。Western印迹分析研究了子痫前期胎盘和孕龄匹配对照组中合胞素的蛋白水平。缺氧对滋养细胞合胞素和ASCT 2表达的影响进一步研究在细胞滋养层细胞培养在2%的氧气（n=7）。主要结果措施合胞素和ASCT 2信使RNA（mRNA）在胎盘组织和细胞滋养层细胞。ASCT 2 mRNA表达自孕中期开始显著下降，此后至孕40周相对稳定。此外，在缺氧条件下培养的先兆子痫胎盘和细胞滋养层细胞中观察到合胞素mRNA表达显着减少，但ASCT 2 mRNA表达并未减少。结论子痫前期患者胎盘中合胞素的表达降低，而ASCT 2的表达不降低，可能与细胞滋养层细胞融合过程改变和胎盘功能紊乱有关。相应地，缺氧降低合胞素，但不ASCT 2基因在培养的细胞滋养层细胞的表达。
Objective To investigate the alterations of syncytin, a fusogenic membrane protein involved in syncytiotrophoblastic layer formation, and its receptor ASCT2 expression in placental development and pre-eclampsia.Design Analyses of syncytin and ASCT2 expression in placentas from different stages of pregnancy and women with pre-eclampsia and in cytotrophoblasts cultured in normoxic and hypoxic conditions.Setting Placental samples were collected from a tertiary medical centre.Population Sixteen women with pre-eclampsia and 58 pregnant women presented as pregnancy (5-19 weeks of gestation) for elective termination, preterm birth, or normal term delivery.Methods The quantitative real-time polymerase chain reaction was used to study the syncytin and ASCT2 expression during placental development in 35 placentas from women without pre-eclampsia (ranged from 5 to 40 weeks of gestation) and the alterations of pre-eclamptic placentas (n=16) compared with gestational-age-matched controls (n=16). Western blot analysis was performed to study the protein level of syncytin in pre-eclamptic placentas and gestational-age-matched controls. The hypoxic effect on trophoblastic syncytin and ASCT2 expression was further studied in cytotrophoblasts cultured in 2% oxygen (n=7).Main outcome measures Syncytin and ASCT2 messenger RNA ( mRNA) in placental tissue and cytotrophoblasts.Results The level of syncytin mRNA expression increased significantly since the first trimester of pregnancy until 37 weeks of gestation, when the level of syncytin expression was reduced. The ASCT2 mRNA expression was decreased significantly since the second trimester and was relatively stable since then to 40 weeks of gestation. Furthermore, a significant reduction in syncytin mRNA expression was observed in pre-eclamptic placentas and cytotrophoblasts cultured in hypoxia, but not a reduction in ASCT2 mRNA expression. Correlatively, the protein level of syncytin was decreased in pre-eclamptic placentas.Conclusions A reduced placental expression of syncytin but not ASCT2 may contribute to altered cytotrophoblastic cell fusion processes and disturbed placental function in pre-eclampsia. Correspondingly, hypoxia decreases syncytin but not ASCT2 gene expression in cultured cytotrophoblasts.