Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.
Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.
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DOI：
10.1529/biophysj.107.112201
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发表时间：
2007-11-01
影响因子：
3.4
通讯作者：
Hell SW
中科院分区：
文献类型：
--
作者：
Egner A;Geisler C;von Middendorff C;Bock H;Wenzel D;Medda R;Andresen M;Stiel AC;Jakobs S;Eggeling C;Schönle A;Hell SW
我们证明了在远场荧光显微镜使用可逆的光开关和本地化的个别荧光团在相对较快的记录速度和完整的细胞内部的纳米级分辨率。通过异步记录单个分子开关周期的光子爆发，这些进步已经成为可能。我们目前的图像从一个完整的哺乳动物细胞的微管网络的分辨率为40 nm。
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.