Rapidly Increasing Prevalence of HIV and Syphilis and HIV-1 Subtype Characterization Among Men Who Have Sex With Men in Jiangsu, China
Rapidly Increasing Prevalence of HIV and Syphilis and HIV-1 Subtype Characterization Among Men Who Have Sex With Men in Jiangsu, China
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中国江苏省男男性行为者中 HIV 和梅毒患病率迅速上升以及 HIV-1 亚型特征
DOI：
10.1097/olq.0b013e31818d3fa0
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发表时间：
2009-02-01
影响因子：
3.1
通讯作者：
Zhang, Chiyu
中科院分区：
文献类型：
--
作者：
Guo, Hongxiong;Wei, Ji-Fu;Zhang, Chiyu
目的：调查中国江苏 2 个城市男男性行为者 (MSM) 的 HIV、乙型肝炎 (HBV)、丙型肝炎 (HCV) 和梅毒的流行情况，并了解该人群中流行的 HIV-1 亚型特征。方法：2006年9月和2007年7月，分别从南京和扬州招募296名MSM和173名MSM。收集血清样本并检测 HIV、HBV、HCV 和梅毒感染。通过RT-巢式PCR和测序确定p17和C2V3区域的核苷酸序列。 HIV-1 亚型通过系统发育分析进行表征。结果：MSM中HIV、HBV、HCV和梅毒感染率分别为5.8%、11.1%、0.7%和27.7%。 2006-2007年江苏省HIV和梅毒患病率明显高于2003年（P≤0.0013），显着高于中国其他地区（P≤0.003）。相比之下，本次研究与 2003 年的研究相比，HBV 和 HCV 患病率没有显着差异（P > 0.05）。 p17的系统发育树显示，HIV-1 B亚型、CRF01_AE和CRF07_BC分别占35.7%、35.7%和28.6%。 C2V3结果显示，分别有45.5%、36.4%和18.2%的序列属于HIV-1 B亚型、CRF01_AE和BC重组体。江苏地区的亚型特征与北京地区存在显着差异（P<0.05）。此外，江苏HIV-1 B毒株与中国大多数B'毒株不同，源自北京。结论：HIV-1流行率快速上升且亚型复杂，提示江苏省MSM迫切需要有效的预防和干预策略。
Objectives: To investigate the prevalence of HIV, hepatitis B (HBV), hepatitis C (HCV), and syphilis among men who have sex with men (MSM) in 2 cities of Jiangsu, China, and to characterize the HIV-1 subtypes prevalent among this population. Methods: During September 2006 and July 2007, 296 and 173 MSM were recruited from Nanjing and Yangzhou, respectively. Sera samples were collected and tested for HIV, HBV, HCV, and syphilis infections. The nucleotide sequences of p17 and C2V3 regions were determined by RT-nested-PCR and sequencing. HIV-1 subtypes were characterized by phylogenetic analysis. Results: The prevalence of HIV, HBV, HCV, and syphilis infections among MSM was 5.8%, 11.1%, 0.7%, and 27.7%, respectively. The prevalence of HIV and syphilis was significantly higher in 2006–2007 than in 2003 (P ≤0.0013) in Jiangsu than in other regions of China (P ≤0.003). In contrast, there was no significant difference in HBV and HCV prevalence between present and 2003 studies (P >0.05). The phylogenetic tree of p17 showed that HIV-1 subtypes B, CRF01_AE, and CRF07_BC accounted for 35.7%, 35.7%, and 28.6%, respectively. The result of C2V3 showed that 45.5%, 36.4%, and 18.2% sequences belonged to HIV-1 subtype B, CRF01_AE, and BC recombinants, respectively. The subtype characterization in Jiangsu was significantly different from those in Beijing (P <0.05). Furthermore, Jiangsu HIV-1 B strains were different from majority of China B′ strains and originated from Beijing. Conclusions: The rapidly increasing prevalence and complex subtypes of HIV-1 suggest that effective prevention and intervention strategies are urgently needed for MSM in Jiangsu.