Subperiosteal implantation of various RF magnetron sputtered Ca-P coatings in goats.
Subperiosteal implantation of various RF magnetron sputtered Ca-P coatings in goats.
复制标题
各种射频磁控管溅射钙磷涂层在山羊骨膜下植入。
DOI：
--
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. A. Jansen
中科院分区：
文献类型：
--
作者：
J. Wolke;J. Wolke;K. D. Groot;J. A. Jansen
本研究的目的是获得更多关于射频磁控溅射磷酸钙（Ca-P）涂层的初始生物事件的信息。因此，将未涂覆和涂覆的椎间盘插入山羊胫骨骨膜下。涂层沉积在商业纯钛上。涂层厚度为0.1或2.0微米。所有的溅射涂层在500℃下进行额外的热处理2小时。在植入1周和3周后，回收实验盘并准备进行组织学和物理化学分析。组织学结果表明，在两个种植周期后，骨膜覆盖了标本。在骨膜和植体之间可见脱细胞层和胶原基质。能谱分析表明，在0.1微米厚的Ca-P涂层中，脱细胞层由C、Ca和P离子组成。在2 μ m厚的Ca-P涂层中也发现了硫酸盐离子的存在。钛盘上只发现了有机物质。此外，扫描电镜显示，即使在注入3周后，两种涂层仍然保持相当大的厚度。薄膜x射线衍射表明，在两个注入周期后，CaP-0.1涂层仍然存在。对样品的红外光谱分析表明，在涂覆的圆盘上形成了与有机相（NH2基团）相关的额外碳酸盐磷灰石（CO3-AP）。基于这些发现，我们得出结论，我们的实验方法非常适合于研究Ca-P涂层周围的愈合过程。此外，我们再次证明了Ca-P材料的初始界面响应不同于钛。
The aim of this study was to obtain more information about the initial biological events around RF magnetron sputtered calcium phosphate (Ca-P) coatings. Therefore, uncoated and coated disks were inserted subperiosteal into the tibia of a goat. The coatings were deposited on commercially pure titanium. The thickness of the coating was 0.1 or 2.0 microm. All the as-sputtered coatings were subjected to an additional heat treatment for 2 h at 500 degrees C. After 1 and 3 weeks of implantation the experimental disks were retrieved and prepared for histological and physicochemical analysis. The histological results demonstrated that the periosteum covered the specimens after both implantation periods. In between the periosteum and implant an acellular layer and a collagen matrix was observed. Energy dispersive spectrometry revealed that the acellular layer consisted of C, Ca, and P ions for the 0.1 microm thick Ca-P coatings. The 2 microm thick Ca-P coatings also showed the presence of sulfate ions in this layer. Only organic material was found on the titanium disks. Further, SEM showed that even after 3-week implantation, a substantial thickness of both coatings was still maintained. Thin film X-ray diffraction demonstrated that after both implantation periods, the CaP-0.1 coating was still present. FTIR of the retrieved specimens demonstrated on the coated disks the formation of additional carbonate apatite (CO3-AP) associated with an organic phase (NH2 groups). On basis of these findings we conclude that our experimental approach is very suitable for the investigation of the healing process around Ca-P coatings. Further, we again demonstrated that the initial interfacial response to Ca-P materials differs from titanium.
羟基磷灰石植入物对大鼠胫骨愈合过程中初级矿化的影响：生化和形态分析。
DOI：
10.1002/jbm.820270808
发表时间：
1993
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Schwartz,Z;Braun,G;Kohavi,D;Brooks,B;Amir,D;Sela,J;Boyan,B
通讯作者：
Boyan,B