Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization.
Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization.
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海胆受精过程中，卵胶会诱导主要精子膜蛋白的去磷酸化。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
V. Vacquier
中科院分区：
文献类型：
--
作者：
G. Ward;V. Vacquier
活体（32）PO(4)（3-）标记显示，斑点Arbacia punctulata精子的160千道尔顿（kDa）蛋白在丝氨酸残基上被组成性磷酸化。将精子暴露于溶解的果冻中会导致该蛋白立即去磷酸化（在5秒内），并同时增加其电泳迁移率（表观分子质量为150 kDa）。在其去磷酸化形式（150 kDa）中，该蛋白是鞭毛质膜的主要成分。事实上，银染聚丙烯酰胺凝胶显示，160/150 kda蛋白质是整个鞭毛中含量第三高的蛋白质。精子在到达卵子表面的过程中必须穿过果冻层。果冻诱导的这种丰富的精子膜蛋白的去磷酸化可能是受精过程中精子和卵子成功相互作用的一个重要事件。
A 160-kilodalton (kDa) protein of Arbacia punctulata sperm is constitutively phosphorylated on serine residues, as shown by in vivo(32)PO(4) (3-) labeling. Exposure of sperm to solubilized egg jelly results in the immediate dephosphorylation (within 5 sec) of this protein and a simultaneous increase in its electrophoretic mobility (to an apparent molecular mass of 150 kDa). In its dephosphorylated form (150 kDa), the protein is a major component of the flagellar plasma membrane. In fact, silver-stained polyacrylamide gels show the 160/150-kDa protein to be the third most abundant protein in the whole flagellum. The spermatozoan must pass through the egg jelly layer on its way to the egg surface. The jelly-induced dephosphorylation of such an abundant sperm membrane protein may be an important event in the successful interaction of sperm and egg during fertilization.
DOI：
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发表时间：
1980
期刊：
Advances in cyclic nucleotide research
影响因子：
--
作者：
Garbers,DL;Kopf,GS
通讯作者：
Kopf,GS