Animal-cell culture media: History, characteristics, and current issues.
Animal-cell culture media: History, characteristics, and current issues.
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DOI：
10.1002/rmb2.12024
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发表时间：
2017-04
影响因子：
3.4
通讯作者：
Asayama Y
中科院分区：
文献类型：
--
作者：
Yao T;Asayama Y
细胞培养技术在一个世纪内得到了广泛的应用，各种培养基被设计出来。本文综述了动物细胞培养基的历史、特点和当前存在的问题。在1880年至2016年5月期间，使用适当的关键词在PubMed和谷歌Scholar上进行文献检索。在细胞培养技术的曙光，培养基的主要成分是自然衍生的产品，如血清。该领域随后逐渐转向使用基于化学的合成介质，因为天然衍生的成分有其缺点，例如批次之间的差异很大。今天，工业上重要的细胞可以在合成培养基中培养。然而，这些成分在这些介质中的组合和浓度仍有待优化。此外，含血清培养基仍普遍用于基础研究领域。在辅助生殖技术和再生医学领域，几乎所有情况下都自然产生一些媒介成分。培养基的进一步改进将有助于减少实验变异，提高生物制药之间的生产效率，改善辅助生殖技术的治疗效果，促进再生医学的实施和普及。
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media. A literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords. At the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances. Further improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.