Core cellular and tissue‐specific mechanisms enable desiccation tolerance in Craterostigma
Core cellular and tissue‐specific mechanisms enable desiccation tolerance in Craterostigma
复制标题
核心细胞和组织特定机制使 Craterostigma 能够耐受干燥
DOI：
10.1111/tpj.16165
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Bartels, Dorothea
中科院分区：
文献类型：
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作者：
VanBuren, Robert;Wai, Ching Man;Giarola, Valentino;Župunski, Milan;Pardo, Jeremy;Kalinowski, Michael;Grossmann, Guido;Bartels, Dorothea
在季节性干旱的地区，复苏植物可以在没有水的情况下存活更长的生命（脱水共生）。这种脱水耐受性需要在空间和时间上协调许多细胞过程，并且单个植物组织面临与其功能相关的独特限制。在这里，我们分析了模式植物Craterostigma（C。车前草），并调查空间和时间的表达动态，以确定遗传因素的基础脱水耐受性。在craterostigmagenome内的同源异型基因具有不同的表达谱，这表明亚基因组对脱水耐受性状的贡献不同。Craterostigmagenome包含近200个串联重复的早期光诱导蛋白，这是耐干燥性的标志性特征，在缺水条件下大量上调。我们在所有组织中确定了干燥反应基因的核心网络，但在恢复过程中观察到每个组织几乎完全独特的表达动态。根和叶具有与光和光保护、自噬和营养物质运输相关的不同反应，反映了它们不同的功能。我们的研究结果强调了一套普遍的可能的祖先干燥耐受机制，以保护细胞大分子脱水下，与组织功能相关的二次适应。
Resurrection plants can survive prolonged life without water (anhydrobiosis) in regions with seasonal drying. This desiccation tolerance requires the coordination of numerous cellular processes across space and time, and individual plant tissues face unique constraints related to their function. Here, we analyzed the complex, octoploid genome of the model resurrection plantCraterostigma(C. plantagineum), and surveyed spatial and temporal expression dynamics to identify genetic elements underlying desiccation tolerance. Homeologous genes within theCraterostigmagenome have divergent expression profiles, suggesting the subgenomes contribute differently to desiccation tolerance traits. TheCraterostigmagenome contains almost 200 tandemly duplicated early light‐induced proteins, a hallmark trait of desiccation tolerance, with massive upregulation under water deficit. We identified a core network of desiccation‐responsive genes across all tissues, but observed almost entirely unique expression dynamics in each tissue during recovery. Roots and leaves have differential responses related to light and photoprotection, autophagy and nutrient transport, reflecting their divergent functions. Our findings highlight a universal set of likely ancestral desiccation tolerance mechanisms to protect cellular macromolecules under anhydrobiosis, with secondary adaptations related to tissue function.