Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii
Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii
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DOI：
10.1002/jobm.200610279
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发表时间：
2007-10-01
影响因子：
3.1
通讯作者：
Sibirny, Andriy A.
中科院分区：
文献类型：
--
作者：
Boretsky, Yuriy R.;Protchenko, Olga V.;Sibirny, Andriy A.
缺铁会导致几种酵母过度合成核黄素，称为产黄素酵母。然而，这种调节的机制尚不清楚。我们发现引起核黄素过量生产和铁过度积累（rib80，rib81和hit1），以及钴过量或铁缺乏的突变都引起了季也蒙毕赤酵母的氧化应激。细胞中的铁含量、季也蒙毕赤酵母野生型和hit1突变株的核黄素和丙二醛产量取决于培养基中使用的碳源类型。这些数据表明，核黄素生物合成和铁同化的调控与细胞的氧化状态有关。
Iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. However, the mechanisms of such regulation are not known. We found that mutations causing riboflavin overproduction and iron hyperaccumulation (rib80, rib81 and hit1), as well as cobalt excess or iron deficiency all provoke oxidative stress in the Pichia guilliermondii yeast. Iron content in the cells, production both of riboflavin and malondialdehyde by P. guilliermondii wild type and hit1 mutant strains depend on a type of carbon source used in cultivation media. The data suggest that the regulation of riboflavin biosynthesis and iron assimilation in P. guilliermondii are linked with cellular oxidative state.