A single gene that promotes interaction of a phytopathogenic bacterium with its insect vector, Drosophila melanogaster
A single gene that promotes interaction of a phytopathogenic bacterium with its insect vector, Drosophila melanogaster
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DOI：
10.1038/sj.embor.embor730
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发表时间：
2003-02-01
期刊：
影响因子：
7.7
通讯作者：
Boccard, F
中科院分区：
文献类型：
--
作者：
Basset, A;Tzou, P;Boccard, F
昆虫是植物和动物疾病的主要媒介，细菌性植物病原体通常由苍蝇传播。我们以前曾报道，一些分离的植物病原细菌物种欧文氏菌carotovora感染果蝇和激活免疫反应。利用遗传筛选，我们现在已经确定了两个基因所需的E。carotovora感染果蝇。其中一个基因具有调节作用，而另一个基因evf赋予感染性表型：它转移到非感染性欧文氏菌菌株或几种肠杆菌中，提高了肠道中的存活率，并引发了免疫反应。欧文氏菌毒力因子（evf）的过表达允许细菌定殖在肠道上皮的顶侧，并且在某些情况下扩散到体腔。我们的研究结果表明，植物病原体和苍蝇之间的特定相互作用，促进其传播。
Insects are major vectors of plant and animal disease, and bacterial phytopathogens are often disseminated by flies. We have previously reported that some isolates of the phytopathogenic bacterial species Erwinia carotovora infect Drosophila and activate an immune response. Using a genetic screen, we have now identified two genes that are required by E. carotovora to infect Drosophila. One of these genes has a regulatory role whereas the other, evf, confers an infectious phenotype: its transfer to non-infectious Erwinia strains or to several enterobacteria improves survival in the gut and triggers the immune response. Overexpression of Erwinia virulence factor (evf) allowed bacteria to colonize the apical side of the gut epithelium and in some cases to spread to the body cavity. Our results demonstrate a specific interaction between plant pathogens and flies that promote their dissemination.